Theoretical bases of 3D Muller-matrix mapping methods for optically anisotropic polycrystalline films of blood are presented. Algorithms for layerwise polarization reconstruction of the parameters of linear and circular birefringence and dichroism are presented. Phase dependencies of the first-fourth-order statistical moments that characterize the parameters of phase and amplitude anisotropy of polycrystalline blood films of healthy and patients with prostate cancer patients are determined. Differential diagnostics of the oncological state with excellent balanced accuracy was realized.
A new method of Stokes-correlational evaluation of polarization-inhomogeneous images of histological sections of optically anisotropic biological tissues of different morphological structure and physiological state was developed. An algorithm for the analytical description and experimental determination of the coordinate distributions of the magnitude of the modulus and the phase of the "two-point" parameters of the Stokes vector is determined. Within the framework of statistical analysis, the possibility of differential diagnosis of weak changes in the optical anisotropy of internal organs tissues of healthy and diabetic rats has been investigated.
The results of Jones matrix mapping of optically thin, non-depolarizing polycrystalline films of synovial fluid (of human joint at reactive and aseptic synovitis) and urine (of healthy donors – patients with albuminuria) are presented. The Jones-matrix model of polarization manifestations of phase anisotropy mechanisms is presented. The method of experimental measurement of coordinate distributions of the values of the modulus and the phase of the Jones matrix elements is suggested. In the framework of statistical and cross-correlation approaches, the maps of the modulus and phase of the Jones-matrix images of optically thin polycrystalline films of human organs with different pathologies are analyzed. A set of objective parameters (statistical and generalized correlation moments) of differential diagnostics of the reactive and aseptic synovitis of the knee joint, as well as of albuminuria was determined with excellent and good balanced precision.
The aim of this study is to study the microscopic, submicroscopic structure and polarization properties of the chordae tendineae of the atrio-ventricular valves of the heart of infants. Methods of laser polarimetry provide additional information about the morphological and optico-anisotropic structure of the biological tissues in norm and in pathology, which is aimed at visualization and in order to receive of the informative images of the polarization-inhomogeneous object fields and their statistical analysis, makes it possible to establish the differential signs of the biological tissues state in norm and pathology. The disordered architectonics of cardiomyocytes of various localization was manifested in false tendons of fibro-muscular and muscular types. The topologically localized and phase-inhomogeneous component of the anisotropic formations of cardiomyocytes manifested itself in the values of statistical moments of higher orders of the Stokes vector-parameter S3. The correlation characteristic of the filtered collagen network indicates an ordered and largescale character of manifestations of hierarchical anisotropy of collagen fibers with a distinct difference in the values of the fourth-order statistical moment (excess) of the values of the ellipticity of the polarization of the image.
The morphological peculiarities of TS mitral valve of the heart of man in normal and abnormal spaced strings of the left ventricle and the study of their structural features depending on the location was studied. Results of statistical dependency and the correlation structure of two-dimensional Mueller matrix elements in different spectral ranges of laser radiation on changes in the distribution of orientations of the optical axes and birefringence of protein crystals biological tissues revealed. Diagnostic sensitivity to changes in optical anisotropic component of biological objects of statistical (statistical moments of the 1st - 4th order) and correlation (half-width of the autocorrelation function and the variance of the power spectra) parameters was founded.
The aim of this research is to clarify the anizotropic structure and polarization properties of the chordae tendinea of the atrio-ventricular heart valves of the foetus.The researches of images of sections of chordae tendinea of the human heart using the method of laser polarimetry have been carried out to systematically analyze the topography of anisotropic properties of collagen fibers, elastic fibers and contractile cardiomyocytes through the obtained intensity distributions at different polarization orientations of Stokes vector parameters, polarization maps, as well as elements of the Mueller matrixes and their respective statistical moments from the 1st to the 4th order. It was shown that the character of manifestations of anisotropic properties manifested itself in different topographical architectures of these types of fibers with a different level of birefringence, which were visualized in different polarization states of the probing and analyzing beams, which was also described by other scientists.
The possibility of solving the inverse problem - extraction of information about linear and circular birefringence and dichroism of light scattering biological layers is considered. An analytical model of the optical anisotropy of depolarizing biological tissues is proposed. The Mueller matrix is represented as a superposition of differential matrices of the first and second orders. Interrelations between the parameters of phase and amplitude anisotropy and elements of a first-order differential matrix are obtained. The algorithms for the experimental measurement of the coordinate distributions of the elements of the polarization component of the Mueller matrix of depolarizing biological tissue are found. The symmetry and features of first-order differential matrices of fibrillar (muscle) and parenchymal (liver) depolarizing biological tissues are investigated. The interrelations between the statistical moments of the first and fourth orders and the features of the morphological polycrystalline structure of the biological tissues of various human organs are found. The ways of application of the differential Mueller-matrix mapping method in clinical diagnostics of the distribution of the phase and amplitude anisotropy distributions are proposed.
A theoretical basis for the method of polarization-interference mapping of optically thin polycrystalline films of human biological fluids is given. The coordinate distributions of the value of the local contrast of the interference distributions of the polarization-inhomogeneous microscopic images of polycrystalline films of the synovial fluid of the human joint are investigated. In the framework of the statistical (statistical moments of the 1st-4th order) approaches, objective criteria for the distribution of the values of local contrast are established. The possibility of differentiation of weak changes in the optical anisotropy of blood films of healthy and patients with breast cancer patients is determined.
The aim of this study is to study the microscopic, submicroscopic structure and polarization properties of the chordae tendineae of the atrio-ventricular valves of the heart of infants. Methods of laser polarimetry provide additional information about the morphological and optico-anisotropic structure of the biological tissues in norm and in pathology, which is aimed at visualization and in order to receive of the informative images of the polarization-inhomogeneous object fields and their statistical analysis, makes it possible to establish the differential signs of the biological tissues state in norm and pathology. The disordered architectonics of cardiomyocytes of various localization was manifested in false tendons of fibro-muscular and muscular types. The topologically localized and phase-inhomogeneous component of the anisotropic formations of cardiomyocytes manifested itself in the values of statistical moments of higher orders of the Stokes vector-parameter S3. The correlation characteristic of the filtered collagen network indicates an ordered and largescale character of manifestations of hierarchical anisotropy of collagen fibers with a distinct difference in the values of the fourth-order statistical moment (excess) of the values of the ellipticity of the polarization of the image.
The morphological peculiarities of TS mitral valve of the heart of man in normal and abnormal spaced strings of the left ventricle and the study of their structural features depending on the location was studied. Results of statistical dependency and the correlation structure of two-dimensional Mueller matrix elements in different spectral ranges of laser radiation on changes in the distribution of orientations of the optical axes and birefringence of protein crystals biological tissues revealed. Diagnostic sensitivity to changes in optical anisotropic component of biological objects of statistical (statistical moments of the 1st - 4th order) and correlation (half-width of the autocorrelation function and the variance of the power spectra) parameters was founded.
The aim of this research is to clarify the anizotropic structure and polarization properties of the chordae tendinea of the atrio-ventricular heart valves of the foetus.The researches of images of sections of chordae tendinea of the human heart using the method of laser polarimetry have been carried out to systematically analyze the topography of anisotropic properties of collagen fibers, elastic fibers and contractile cardiomyocytes through the obtained intensity distributions at different polarization orientations of Stokes vector parameters, polarization maps, as well as elements of the Mueller matrixes and their respective statistical moments from the 1st to the 4th order. It was shown that the character of manifestations of anisotropic properties manifested itself in different topographical architectures of these types of fibers with a different level of birefringence, which were visualized in different polarization states of the probing and analyzing beams, which was also described by other scientists.
A new model of the correlation description of the optically anisotropic component of biological tissues is proposed. To characterize the transformation of the fibrillar network of laser radiation, a new parameter is used-the complex "twopoint" Stokes vector. The interconnections between the parameters of the "two-point" Stokes vector with the distributions of the optical axes and birefringence of the fibrillar network of biological tissue are found. A scheme of generalized Stokes polarimetry of microscopic images of histological sections of fibrillar biological tissues is developed. A technique for direct polarization measurement of the distributions of the real and imaginary parts of the "two-point" Stokes vector is proposed. Maps of phase distributions of the "two-point" Stokes vector of histological sections of the myocardium and endometrium. The sensitivity, specificity and balanced accuracy of the method of polarizationcorrelation mapping of fibrillar networks of biological crystals are determined. Within the framework of the statistical analysis of the phase maps of the "two-point" Stokes vector, histological sections of the myocardium and endometrium, objective criteria for diagnosing endometrial cancer have been found and an excellent balanced accuracy of the cause of death.
A model of weak phase fluctuations of polycrystalline films of biological fluids is proposed. A correlation approach has been used to describe the polarization manifestations of the linear and circular birefringence of biological planar polycrystalline networks. Algorithms of polarization experimental measurement of the module (orientation map) and phase (phase map) of a set of "two-point" parameters of the Stokes vector are determined. The sets of orientation and phase maps of polycrystalline films of bile and blood are studied experimentally. The diagnostic possibilities of statistical analysis of the module and phase distributions of the "two-point" parameters of the Stokes vector of polarization-inhomogeneous images are considered. The magnitudes and ranges of changes in the set of statistical moments of the 1st and 4th orders that characterize the orientation and phase maps of polycrystalline films of bile and blood are found. The sensitivity, specificity and balanced accuracy of the method of polarization-correlation mapping in the diagnosis of early stage of cholelithiasis, as well as differentiation of the degree of blood losses, were determined.
The morphological and anizotropy characteristics of the left ventricle false chordae tendineae of human heart in the aspect of their anisotropic properties using spectroscopic-polarization methods was studied. There are given the results of statistical correlation structure of the spectral dependence of the two-dimensional Mueller matrix elements and phase shifts of histological sections of different morphological structure and physiological state. The relationship between the distribution of orientations of the optical axes birefringent miozyn fibrils with a set of statistical moments that characterize the distributions of Mueller matrix elements in different spectral ranges and half-width corresponding autocorrelation functions are established.
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