Multidrug-resistant bacteria stem from the massive use of non-specific antibiotics prescribed to manage bacterial infections. The therapeutic use of viruses called bacteriophages is a promising complementary and personalised strategy requiring very accurate phage selection for patient administration. Therefore, we are developing an interdisciplinary methodology for phage susceptibility testing (PST) based on bio-photonic microsystems. We demonstrated the use of on-chip optical photonic crystals on silicon-on-insulator (SOI) for the spatial confinement of bacteria and phages. We will present our state of the art of this project and the methods currently used to study the interactions between SOI and biological objects.
We will show results towards a new method to perform antibiotic or phage susceptibility test with single phages or bacteria trapped in an optical chip. The system used is a photonic chip containing photonic crystal hollow cavities topped by a microfluidic system allowing the transport of bacteria and phages. We will report the optical signature of the trapping of bacteria and phages in the transmitted light exciting the optical trap. This allows the distinction of different phages families as well as the level of stress or death of a single bacteria in the presence of antibiotics or phages.
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