Prof. Paul M. W. French Profile

Prof. Paul M. W. French

Professor at Imperial College London

SPIE Involvement:

Conference Program Committee | Author

Publications (57)

Proceedings Article | 7 December 2021 Paper

Single-shot volumetric imaging using optical projection tomography

Connor Darling, Samuel P. Davis, Sunil Kumar, Paul M. French, James McGinty

Proceedings Volume 11922, 119220J (2021) https://doi.org/10.1117/12.2615706

KEYWORDS: Cameras, Tomography, Projection systems, Optical tomography, Data acquisition, Stereoscopy, Optical imaging, Objectives, Lenses, Compressed sensing

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Proceedings Article | 7 December 2021 Paper

Robust optical autofocus system utilizing neural networks applied to automated multiwell plate STORM microscopy

J. Lightley, F. Görlitz, S. Kumar, R. Kalita, A. Kolbeinsson, E. Garcia, Y. Alexandrov, V. Bousgouni, R. Wysoczanski, P. Barnes, L.. Donelly, C. Bakal, C. Dunsby, M. A. Neil, S. Flaxman, P. M. French

Proceedings Volume 11922, 1192205 (2021) https://doi.org/10.1117/12.2615663

KEYWORDS: Microscopes, Data acquisition, Microscopy, Data modeling, Imaging systems, Objectives, Neural networks, Infrared radiation, Infrared imaging, Cameras

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Proceedings Article | 7 December 2021 Paper

Single-shot quantitative phase contrast using polarisation-resolved differential phase microscopy

R. Kalita, J. Lightley, S. Kumar, Y. Alexandrov, E. Garcia, W. Flanagan, M. A. Neil, C. Dunsby, P. M. French

Proceedings Volume 11922, 119220Y (2021) https://doi.org/10.1117/12.2615736

KEYWORDS: Microscopy, Phase contrast, LCDs, Cameras, Polarizers, Microscopes, Luminescence, Optical filters, Objectives, Image acquisition

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Proceedings Article | 3 March 2020 Paper

Towards easier, faster, super-resolved microscopy

Frederik Görlitz, Riccardo Wysoczanski, Sunil Kumar, Jonathan Lightley, Edwin Garcia, Yuriy Alexandrov, Ian Munro, Simon Johnson, Martin Kehoe, Callum Hollick, Jeremy Graham, Louise Donnelly, Peter Barnes, Christopher Dunsby, Mark Neil, Paul M. French

Proceedings Volume 11246, 112460R (2020) https://doi.org/10.1117/12.2550682

KEYWORDS: Microscopy, Microscopes, Stimulated emission depletion microscopy, Spatial light modulators, Objectives, Luminescence, Holograms, Data processing, Point spread functions, Data acquisition

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Proceedings Article | 2 March 2020 Paper

FLIM, FRET and high content analysis

Edwin Garcia, Wenjun Guo, Sunil Kumar, Frederik Görlitz, Hugh Sparks, Yuriy Alexandrov, Ian Munro, Douglas Kelly, Sean Warren, George Chennell, Alessandro Sardini, David Carling, Peter Thorpe, Christopher Dunsby, Paul M. French

Proceedings Volume 11244, 1124417 (2020) https://doi.org/10.1117/12.2547517

KEYWORDS: Fluorescence lifetime imaging, Fluorescence resonance energy transfer, Biosensors, Proteins, Luminescence, Data acquisition, Confocal microscopy, Fluorescent proteins, Microscopes, Biological research

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Proceedings Article | 22 July 2019 Paper

Automated multiwell plate STORM: towards open source super-resolved high content analysis

F. Görlitz, J. Lightley, S. Kumar, E. Garcia, M. Yan, R. Wysoczanski, Y. Alexandrov, J. Baker, P. Barnes, I. Munro, L. Donnelly, C. Dunsby, M. A. Neil, P. M. French

Proceedings Volume 11076, 1107605 (2019) https://doi.org/10.1117/12.2526940

KEYWORDS: Microscopy, Data processing, Data acquisition, Imaging systems, Microscopes

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Proceedings Article | 4 March 2019 Presentation

Exploiting patterned illumination and detection in optical projection tomography (Conference Presentation)

Samuel Davis, Sunil Kumar, Laura Wisniewski, Marie-Christine Ramel, Laurence Bugeon, Teresa Correia, Maggie Dallman, Simon Arridge, Paul Frankel, James McGinty, PaulM. French

Proceedings Volume 10883, 108830M (2019) https://doi.org/10.1117/12.2508653

KEYWORDS: Projection systems, Optical tomography, Tomography, Light scattering, Scattering, X-ray optics, X-ray computed tomography, Signal attenuation, Luminescence, Organisms

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Optical Projection Tomography (OPT), the optical equivalent of x-ray computed tomography, reconstructs the 3D structure of a sample from a series of wide-field 2D projections acquired at different angles [1]. OPT is used to map the optical attenuation and/or fluorescence distributions of intact transparent samples without the need for mechanical sectioning. While it is typically applied to chemically cleared samples, it can also be used to image inherently transparent or weakly scattering live organisms including adult zebrafish up to ~1cm in diameter [2]. When applying OPT to live samples it is important to minimise the data acquisition time while maximising the image quality in the presence of scattering. The former issue can be addressed using compressive sensing to reduce the number of projections required [3]. Scattered light can be rejected using structured illumination [4], but this removes emission from regions the excitation modulation does not reach and reduces the available dynamic range. To address this, we have explored the rejection of scattered light by acquiring projections with parallel semi-confocal line illumination and detection in an approach we describe as slice-OPT (sl-OPT). The impact of optical scattering can also be reduced by imaging at longer wavelengths [5]. We are exploring OPT in the NIR 1&2 spectral windows. However, exotic array detectors, e.g. for short wave infrared light, are costly and so we are also developing a single pixel camera [6] approach. We will present our progress applying these techniques to 3D imaging of vasculature and tumour burden in live adult zebrafish. [1] Sharpe et al, Science, vol. 296, Issue 5567, pp. 541-545, 2002. [2] Kumar et al, Oncotarget, vol. 7, no.28, pp. 43939-43948, 2016. [3] Correia et al, PloS one, vol. 10, no. 8, p. e0136213, 2015. [4] Kristensson et al, Optics express, vol. 20, no. 13, pp. 14437-14450, 2012. [5] Shi et al., Journal of Biophotonics, vol. 9, no. 1-2, pp. 38-43, 2016. [6] Duarte et al., IEEE Signal Processing Magazine, vol. 25, no. 2, pp. 83-91, 2008.

Proceedings Article | 24 April 2017 Presentation

Functional imaging of live Zebrafish using fluorescence lifetime optical projection tomography (Conference Presentation)

Natalie Andrews, Samuel Davis, Carys Hay, Sunil Kumar, Marie-Christine Ramel, Laurence Bugeon, James McGinty, Margaret Dallman, Paul M. French

Proceedings Volume 10068, 1006808 (2017) https://doi.org/10.1117/12.2252721

KEYWORDS: X-ray optics, Luminescence, Fluorescence lifetime imaging, Fluorescence resonance energy transfer, Biosensors, 3D image reconstruction, Functional imaging, Projection systems, Optical tomography, Tomography

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Proceedings Article | 27 April 2016 Presentation

Three-dimensional fluorescence imaging by stage-scanning oblique plane microscopy (Conference Presentation)

Vincent Maioli, Frederik Görlitz, Sean Warren, Sunil Kumar, Paul M. French, George Chennell, Alessandro Sardini, David Carling, Frederike Alwes, Christopher Dunsby

Proceedings Volume 9713, 971318 (2016) https://doi.org/10.1117/12.2208808

KEYWORDS: 3D image processing, Microscopy, 3D acquisition, Luminescence, Microscopes, Cameras, Fluorescence resonance energy transfer, Stereoscopy, Objectives, Tumors

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Proceedings Article | 19 November 2014 Paper

Nanoparticle-assisted STED, theory, and experimental demonstration (presentation video)

Yannick Sonnefraud, Yonatan Sivan, Hugo Sinclair, Christopher Dunsby, Mark Neil, Paul French, Stefan Maier

Proceedings Volume 9169, 916903 (2014) https://doi.org/10.1117/12.2060993

KEYWORDS: Stimulated emission depletion microscopy, Video, Metals, Nanoparticles, Super resolution, Luminescence, Particles, Nanoimaging, Nanospectroscopy, Current controlled current source

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Proceedings Article | 17 March 2014 Paper

Autofluorescence lifetime metrology for label-free detection of cartilage matrix degradation

Mohammad Nickdel, João Lagarto, Douglas Kelly, Hugh Manning, Kazuhiro Yamamoto, Clifford Talbot, Christopher Dunsby, Paul French, Yoshifumi Itoh

Proceedings Volume 8940, 89400H (2014) https://doi.org/10.1117/12.2037285

KEYWORDS: Cartilage, Collagen, Tissues, Fluorescence lifetime imaging, Luminescence, Fluorometers, Scanning electron microscopy, Microscopy, Metrology, Microscopes

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Proceedings Article | 8 May 2012 Paper

Towards gene therapy based on femtosecond optical transfection

M. Antkowiak, M. Torres-Mapa, J. McGinty, M. Chahine, L. Bugeon, A. Rose, A. Finn, S. Moleirinho, K. Okuse, M. Dallman, P. French, S. Harding, P. Reynolds, F. Gunn-Moore, K. Dholakia

Proceedings Volume 8427, 84270R (2012) https://doi.org/10.1117/12.922835

KEYWORDS: Neurons, Femtosecond phenomena, In vivo imaging, Luminescence, Objectives, Tissues, Heart, Laser optics, Medicine, Plasma

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Proceedings Article | 14 June 2011 Paper

Non-invasive imaging of skin cancer with fluorescence lifetime imaging using two photon tomography

Rakesh Patalay, Clifford Talbot, Yuriy Alexandrov, Ian Munro, Hans Georg Breunig, Karsten König, Sean Warren, Mark Neil, Paul M. French, Anthony Chu, Gordon Stamp, Christopher Dunsby

Proceedings Volume 8087, 808718 (2011) https://doi.org/10.1117/12.889314

KEYWORDS: Luminescence, Fluorescence lifetime imaging, Skin, Tissues, Hyperspectral imaging, Diagnostics, In vivo imaging, Spectroscopy, Skin cancer, Image segmentation

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Proceedings Article | 1 March 2011 Paper

An automated wide-field time-gated optically sectioning fluorescence lifetime imaging multiwell plate reader for high-content analysis of protein-protein interactions

Dominic Alibhai, Sunil Kumar, Douglas Kelly, Sean Warren, Yuriy Alexandrov, Ian Munro, James McGinty, Clifford Talbot, Edward Murray, Frank Stuhmeier, Mark A. Neil, Chris Dunsby, Paul M. French

Proceedings Volume 7904, 79041Q (2011) https://doi.org/10.1117/12.875135

KEYWORDS: Fluorescence resonance energy transfer, Fluorescence lifetime imaging, Luminescence, Proteins, Data modeling, Picosecond phenomena, Image segmentation, Rhodamine B, Plasma, Biological research

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Proceedings Article | 1 March 2011 Paper

A multispectral FLIM tomograph for in-vivo imaging of skin cancer

C. Talbot, R. Patalay, I. Munro, H. Breunig, K. König, Y. Alexandrov, S. Warren, A. Chu, G. Stamp, M. A. Neil, Paul M. French, Christopher Dunsby

Proceedings Volume 7903, 79032B (2011) https://doi.org/10.1117/12.873567

KEYWORDS: Sensors, Fluorescence lifetime imaging, Skin, Luminescence, In vivo imaging, Image segmentation, Spectroscopy, Image sensors, Detection and tracking algorithms, Hyperspectral imaging

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Proceedings Article | 1 March 2011 Paper

A STED-FLIM microscope applied to imaging the natural killer cell immune synapse

M. Lenz, A. C. Brown, E. Auksorius, D. Davis, C. Dunsby, M. A. Neil, P. M. French

Proceedings Volume 7903, 79032D (2011) https://doi.org/10.1117/12.875018

KEYWORDS: Stimulated emission depletion microscopy, Fluorescence lifetime imaging, Microscopes, Confocal microscopy, Microscopy, Luminescence, Spatial resolution, Sapphire lasers, Structured optical fibers, Spatial light modulators

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Proceedings Article | 22 February 2011 Paper

Fluorescence lifetime imaging endoscopy

G. Kennedy, S. Coda, A. Thompson, D. Elson, M. A. Neil, G. Stamp, A. Thillainayagam, B. Viellerobe, F. Lacombe, C. Dunsby, Paul M. French

Proceedings Volume 7893, 789308 (2011) https://doi.org/10.1117/12.874968

KEYWORDS: Fluorescence lifetime imaging, Endoscopes, Luminescence, Confocal microscopy, Endoscopy, Tissues, Biopsy, In vivo imaging, Tissue optics, Fluorescence resonance energy transfer

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Proceedings Article | 18 February 2011 Paper

Fluorescence lifetime imaging of skin cancer

Rakesh Patalay, Clifford Talbot, Ian Munro, Hans Georg Breunig, Karsten König, Yuri Alexandrov, Sean Warren, Mark Neil, Paul M. French, Anthony Chu, Gordon Stamp, Chris Dunsby

Proceedings Volume 7883, 78830A (2011) https://doi.org/10.1117/12.873298

KEYWORDS: Luminescence, Fluorescence lifetime imaging, Skin cancer, Tissues, Skin, Photons, Two photon excitation microscopy, Diagnostics, Photonics, Roads

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Proceedings Article | 17 February 2011 Paper

Hyperspectral fluorescence lifetime fibre probe spectroscopy for use in the study and diagnosis of osteoarthritis and skin cancer

Alex Thompson, Hugh Manning, Mikkel Brydegaard, Sergio Coda, Gordon Kennedy, Rakesh Patalay, Ulrika Waitong-Braemming, Pieter De Beule, Mark Neil, Stefan Andersson-Engels, Yoshifumi Itoh, Niels Bendsøe, Christopher Dunsby, Katarina Svanberg, Paul M. French

Proceedings Volume 7895, 78950G (2011) https://doi.org/10.1117/12.875120

KEYWORDS: Luminescence, Spectroscopy, Cartilage, Skin cancer, Tissues, In vivo imaging, Collagen, Tissue optics, Reflectivity, Diffuse reflectance spectroscopy

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Proceedings Article | 27 February 2010 Paper

Investigating fast enzyme-DNA kinetics using multidimensional fluorescence imaging and microfluidics

Tom Robinson, Hugh Manning, Christopher Dunsby, Mark A. A. Neil, Geoff Baldwin, Andrew de Mello, Paul M. W. French

Proceedings Volume 7593, 759304 (2010) https://doi.org/10.1117/12.840035

KEYWORDS: Microfluidics, Luminescence, Anisotropy, Fluorescence lifetime imaging, Fluorescence anisotropy, Polarization, Microscopes, Confocal microscopy, Computer simulations, Computational fluid dynamics

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Proceedings Article | 26 February 2010 Paper

Tomographic imaging of flourescence resonance energy transfer in highly light scattering media

Vadim Soloviev, James McGinty, Khadija Tahir, Romain Laine, Daniel Stuckey, P. Surya Mohan, Joseph Hajnal, Alessandro Sardini, Paul M. French, Simon Arridge

Proceedings Volume 7573, 75730G (2010) https://doi.org/10.1117/12.840522

KEYWORDS: Fluorescence resonance energy transfer, Light scattering, Tomography, Luminescence, Resonance energy transfer, Quantum efficiency, Proteins, Fluorescence lifetime imaging, Scattering, Calcium

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Proceedings Article | 24 February 2010 Paper

tomoFLIM: fluorescence lifetime projection tomography

James McGinty, Daniel Stuckey, Khadija Tahir, Romain Laine, Joseph Hajnal, Alessandro Sardini, Paul French

Proceedings Volume 7570, 75700G (2010) https://doi.org/10.1117/12.841753

KEYWORDS: Luminescence, Fluorescence resonance energy transfer, Calcium, Tomography, Reconstruction algorithms, Fluorescence lifetime imaging, Head, 3D modeling, 3D image processing, Fluorescent proteins

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Optical Projection Tomography (OPT) is a wide-field technique for measuring the threedimensional distribution of absorbing/fluorescing species in non-scattering (optically cleared) samples up to ~1cm in size, and as such is the optical analogue of X-ray computed tomography. We have extended the intensity-based OPT technique to measure the three-dimensional fluorescence lifetime distribution (tomoFLIM) in transparent samples. Due to its inherent ratiometric nature, fluorescence lifetime measurements are robust against intensity-based artifacts as well as producing a quantitative measure of the fluorescence signal, making it particularly suited to Förster Resonance Energy Transfer (FRET) measurements. We implement tomoFLIM via OPT by acquiring a series of wide-field time-gated images at different relative time delays with respect to a train of excitation pulses for a range of projection angles. For each time delay, the three-dimensional time-gated intensity distribution is reconstructed using a filtered back projection algorithm and the fluorescence lifetime is subsequently determined for each reconstructed horizontal plane by iterative fitting of an appropriate decay model. We present a tomographic reconstruction of a fluorescence lifetime resolved FRET calcium contruct, TN-L15 cytosol suspension, in a silicone phantom. This genetically encoded sensor, TN-L15, comprises the calcium-binding domain of Troponin C, flanked by the fluorophores cyan fluorescent protein and citrine. In the presence of calcium ions TN-L15 changes conformation bringing the two fluorophores into close proximity, resulting in FRET. We also present autofluorescence and fluorescently labelled tomoFLIM reconstructions of chick embryos, including a genetically encoded fluorophore TagRFP-T. The fluorophore was electroporated in ovo into the neural tube of the embryos, which were subsequently dissected two days post-electroporation, fixed in ethanol and optically cleared for OPT/tomoFLIM acquisition. The reconstructed 3-D fluorescence lifetime image provides contrast between the genetically labelled TagRFP-T and the emitted autofluorescence.

Proceedings Article | 13 July 2007 Paper

Applications of rapid time-gated hyperspectral FLIM: live cell imaging of membrane order and 6-D microscopy

H. Manning, D. Owen, E. Auksorius, P. de Beule, S. Oddos, C. Talbot, C. Dunsby, I. Munro, A. Magee, M. Neil, P. French

Proceedings Volume 6630, 663019 (2007) https://doi.org/10.1117/12.728479

KEYWORDS: Luminescence, Fluorescence lifetime imaging, Microscopes, Data acquisition, Charge-coupled devices, Live cell imaging, Microscopy, Confocal microscopy, Tissues, Spectral resolution

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Proceedings Article | 12 July 2007 Paper

Fluorescence lifetime imaging through turbid media reconstructed in the Fourier domain using time-gated imaging data

Vadim Soloviev, Khadija Tahir, James McGinty, Dan Elson, Mark Neil, Alessandro Sardini, Joseph Hajnal, Simon Arridge, Paul French

Proceedings Volume 6629, 66291V (2007) https://doi.org/10.1117/12.728481

KEYWORDS: Luminescence, Scattering, Quantum efficiency, Fluorescence lifetime imaging, CCD cameras, Picosecond phenomena, Fourier transforms, Tomography, Cameras, Epoxies

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Proceedings Article | 19 February 2007 Paper

Development of a hyperspectral fluorescence lifetime imaging microscope and its application to tissue imaging

Dylan Owen, Hugh Manning, Pieter de Beule, Clifford Talbot, Jose Requejo-Isidro, Chris Dunsby, James McGinty, Richard Benninger, Dan Elson, Ian Munro, Neil Galletly, M. Jon Lever, Gordon Stamp, Praveen Anand, Mark Neil, Paul French

Proceedings Volume 6441, 64411K (2007) https://doi.org/10.1117/12.700081

KEYWORDS: Fluorescence lifetime imaging, Luminescence, Microscopes, Tissues, Confocal microscopy, Hyperspectral imaging, Spectrographs, Cameras, Tissue optics, Sensors

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Proceedings Article | 14 February 2007 Paper

A novel hyperspectral lifetime probe for autofluorescence

Pieter De Beule, C. Dunsby, D. Owen, N. Galletly, U. Anand, C. Benham, A. Naylor, G. Stamp, P. Anand, P. French

Proceedings Volume 6433, 643303 (2007) https://doi.org/10.1117/12.700590

KEYWORDS: Luminescence, Skin, Proteins, Ultraviolet radiation, Optical filters, Mode conditioning cables, Tissues, Optical fibers, Sensors, Roads

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Proceedings Article | 14 February 2007 Paper

Fluorescence lifetime imaging using light-emitting diodes

Gordon Kennedy, Daniel Elson, Ian Munro, Vincent Poher, Paul French, Mark Neil

Proceedings Volume 6443, 644315 (2007) https://doi.org/10.1117/12.701088

KEYWORDS: Light emitting diodes, Fluorescence lifetime imaging, Luminescence, Modulation, Picosecond phenomena, Microscopy, LED lighting, Digital filtering, Light sources and illumination, Confocal microscopy

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Proceedings Article | 14 February 2007 Paper

Microscopy using micropixellated light emitting diodes

V. Poher, G. Kennedy, D. Elson, P. French, M. Neil, H. Z. Zhang, E. Gu, Z. Gong, C. Griffin, J. Girkin, M. Dawson

Proceedings Volume 6443, 644313 (2007) https://doi.org/10.1117/12.700811

KEYWORDS: Light emitting diodes, Microscopy, Confocal microscopy, Microscopes, Light sources, Line scan image sensors, Luminescence, Solid state electronics, CCD cameras, Cameras

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Proceedings Article | 8 October 2005 Paper

Fluorescence lifetime imaging microscopy using a tunable continuum source and a Nipkow disk confocal microscope

D. Grant, E. Auksorius, D. Schimpf, D. Elson, C. Dunsby, J. Requejo-Isidro, I. Munro, M. Neil, P. French, P. Courtney

Proceedings Volume 5860, 58600K (2005) https://doi.org/10.1117/12.632952

KEYWORDS: Fluorescence lifetime imaging, Confocal microscopy, Microscopes, Microscopy, Luminescence, Photonic crystal fibers, Confocal fluorescent microscopy, Multiphoton microscopy, Physics, Crystals

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Proceedings Article | 8 October 2005 Paper

Application of hyperspectral fluorescence lifetime imaging to tissue autofluorescence: arthritis

C. Talbot, R. Benninger, P. de Beule, J. Requejo-Isidro, D. Elson, C. Dunsby, I. Munro, M. Neil, A. Sandison, N. Sofat, H. Nagase, P. French, M. Lever

Proceedings Volume 5862, 58620T (2005) https://doi.org/10.1117/12.633034

KEYWORDS: Cartilage, Tissues, Fluorescence lifetime imaging, Luminescence, Head, Hyperspectral imaging, Physics, Software development, Diagnostics, Statistical analysis

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SPIE Journal Paper | 1 September 2005 Open Access

Toward the clinical application of time-domain fluorescence lifetime imaging

Ian Munro, James McGinty, Neil Galletly, Jose Requejo-Isidro, Peter Lanigan, Daniel Elson, Christopher Dunsby, Mark Neil, M. John Lever, Gordon Stamp, Paul French

JBO, Vol. 10, Issue 05, 051403, (September 2005) https://doi.org/10.1117/12.10.1117/1.2102807

KEYWORDS: Fluorescence lifetime imaging, Luminescence, Tissues, Endoscopes, Tissue optics, Endoscopy, Signal to noise ratio, Charge-coupled devices, Picosecond phenomena, Image intensifiers

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Proceedings Article | 1 September 2005 Paper

Video rate fluorescence lifetime imaging and fluorescence lifetime endoscopy

J. McGinty, I. Munro, J. Requejo-Isidro, D. Elson, C. Dunsby, M. Neil, P. French, N. Galletly, G. Stamp

Proceedings Volume 5864, 586408 (2005) https://doi.org/10.1117/12.632913

KEYWORDS: Fluorescence lifetime imaging, Endoscopes, Luminescence, Endoscopy, Tissues, Tissue optics, Video, Picosecond phenomena, Biomedical optics, Laparoscopy

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Proceedings Article | 23 September 2003 Paper

Photorefractive holography for real-time coherence gated imaging

Christopher Dunsby, Y. Gu, Z. Ansari, D. Nolte, M. Melloch, P. French

Proceedings Volume 5067, (2003) https://doi.org/10.1117/12.518500

KEYWORDS: Coherence (optics), Holography, Gated imaging, Signal detection, Video, Scattering media, Cameras, Charge-coupled devices, Optical coherence tomography, Physics

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Proceedings Article | 8 July 2003 Paper

Wide-field coherence gated imaging: photorefractive holography and wide-field coherent heterodyne imaging

Christopher Dunsby, Yan Gu, David Nolte, Michael Melloch, Paul French

Proceedings Volume 4956, (2003) https://doi.org/10.1117/12.478993

KEYWORDS: Coherence imaging, Light scattering, Charge-coupled devices, Holography, CCD cameras, Scattering, Polarization, Coherence (optics), Optical coherence tomography, Signal detection

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Proceedings Article | 8 July 2003 Paper

Imaging of tumor necroses using full-frame optical coherence imaging

Ping Yu, L. Peng, M. Mustata, David Nolte, J. Turek, Michael Melloch, Christopher Dunsby, Yan Gu, Paul French

Proceedings Volume 4956, (2003) https://doi.org/10.1117/12.477823

KEYWORDS: Tumors, Holograms, Holography, Coherence imaging, Speckle, Tissue optics, Optical coherence tomography, CCD cameras, Four wave mixing, Biomedical optics

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Proceedings Article | 14 June 2002 Paper

Optical coherence imaging of rat tumor spheroids

Ping Yu, M. Mustata, William Headley, David Nolte, J. Turek, Paul French

Proceedings Volume 4619, (2002) https://doi.org/10.1117/12.470485

KEYWORDS: Tumors, Holograms, Coherence imaging, Optical coherence tomography, Four wave mixing, Video, Beam splitters, Biomedical optics, Holography, Cameras

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Proceedings Article | 14 June 2002 Paper

Low-coherence photorefractive holography for high-speed 3D imaging including through scattering media

Christopher Dunsby, Y. Gu, Zunaira Ansari, Duncan Parsons-Karavassilis, Jan Siegel, Masahide Itoh, S. Iwamoto, William Headley, Michael Melloch, David Nolte, Paul French

Proceedings Volume 4619, (2002) https://doi.org/10.1117/12.470469

KEYWORDS: Microscopes, Holography, Light scattering, Coherence (optics), Modulation, Stereoscopy, Scattering, CCD cameras, Charge-coupled devices, Mirrors

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Proceedings Article | 11 June 2002 Paper

Autocorrelation imaging of 3D structures using a femtosecond laser: application to imaging of sandstone

Ping Yu, M. Mustata, William Headley, L. Pyrak-Nolte, David Nolte, Paul French

Proceedings Volume 4643, (2002) https://doi.org/10.1117/12.470423

KEYWORDS: Holograms, Coherence imaging, Light scattering, Optical coherence tomography, Scanning electron microscopy, Translucency, Holography, Geometrical optics, Reflection, 3D image processing

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Proceedings Article | 31 May 2002 Paper

High-speed 3-D imaging using photorefractive holography with novel low-coherence interferometers

Y. Gu, Zunaira Ansari, Christopher Dunsby, Mary Tziraki, Duncan Parsons-Karavassilis, Jan Siegel, Paul French, David Nolte, Michael Melloch

Proceedings Volume 4705, (2002) https://doi.org/10.1117/12.469019

KEYWORDS: Holography, Light scattering, Stereoscopy, 3D image reconstruction, Luminescence, Microscopes, Coherence (optics), Scattering media, Beam splitters, Prisms

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Proceedings Article | 2 November 2001 Paper

Five-dimensional fluorescence microscopy

Stephen Webb, D. Elson, Jan Siegel, Sandrine Leveque-Fort, Y. Gu, Duncan Parsons-Karavassilis, Mary Cole, Paul French, M. Lever, Leon Sucharov, Mark Neil, Rimas Juskaitis, Tony Wilson

Proceedings Volume 4431, (2001) https://doi.org/10.1117/12.447405

KEYWORDS: Luminescence, Fluorescence lifetime imaging, Imaging systems, Tissues, Microscopy, Optical spheres, Tissue optics, Biomedical optics, Microscopes, Multispectral imaging

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Proceedings Article | 2 November 2001 Paper

Application of the stretched exponential function to fluorescence lifetime imaging of biological tissue

Jan Siegel, K.C. Lee, Stephen Webb, Sandrine Leveque-Fort, Mary Cole, Richard Jones, Keith Dowling, Paul French, M. Lever

Proceedings Volume 4431, (2001) https://doi.org/10.1117/12.447407

KEYWORDS: Fluorescence lifetime imaging, Tissues, Luminescence, Data modeling, Collagen, Proteins, Statistical modeling, Tissue optics, Mathematical modeling, Image quality

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Proceedings Article | 24 October 2001 Paper

High-speed 3D imaging using photorefractive holography with novel low-coherence interferometers

Yan Gu, Zunaira Ansari, Jan Siegel, Christopher Dunsby, Masahide Itoh, Duncan Parsons-Karavassilis, Mary Tziraki, Richard Jones, Keith Dowling, Paul French, David Nolte, William Headley, Michael Melloch

Proceedings Volume 4434, (2001) https://doi.org/10.1117/12.446694

KEYWORDS: Holography, Light scattering, Modulation, Semiconductor lasers, Light emitting diodes, Imaging systems, Tissue optics, CCD cameras, Stereoscopy, Luminescence

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Proceedings Article | 13 April 2001 Paper

High-speed 3D imaging using photorefractive holography

Yan Gu, Zunaira Ansari, Duncan Parsons-Karavassilis, Paul French, David Nolte, Michael Melloch

Proceedings Volume 4298, (2001) https://doi.org/10.1117/12.424903

KEYWORDS: Holography, Imaging systems, Light scattering, Light emitting diodes, Holograms, 3D image processing, Stereoscopy, Semiconductor lasers, Image acquisition, Scattering

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Proceedings Article | 22 November 2000 Paper

Time-gated holographic imaging using photorefractive media

Zunaira Ansari, Yan Gu, Mary Tziraki, Duncan Parsons-Karavassilis, Richard Jones, Paul French, David Nolte, Michael Melloch

Proceedings Volume 4160, (2000) https://doi.org/10.1117/12.407606

KEYWORDS: Holography, Light emitting diodes, Optical coherence tomography, Holograms, Coherence (optics), Prisms, Laser sources, Laser scattering, Scattering, Light scattering

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Proceedings Article | 6 April 2000 Paper

Whole-field coherent imaging through turbid media using photorefractive holography

Mary Tziraki, Richard Jones, Paul French, K. Kwolek, David Nolte, Michael Melloch

Proceedings Volume 4001, (2000) https://doi.org/10.1117/12.381483

KEYWORDS: Holography, Holograms, Scattering, Tissues, Video, Speckle, Light emitting diodes, 3D image reconstruction, Imaging systems, Light scattering

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Proceedings Article | 4 June 1999 Paper

Fluorescence lifetime imaging for biomedicine using all-solid state ultrafast laser technology

Richard Jones, Keith Dowling, Mary Cole, Duncan Parsons-Karavassilis, Paul French, M. Lever, Jonathan Hares, Anthony Dymoke-Bradshaw

Proceedings Volume 3616, (1999) https://doi.org/10.1117/12.351824

KEYWORDS: Fluorescence lifetime imaging, Luminescence, Tissues, Amplifiers, Laser ablation, Picosecond phenomena, Imaging systems, Laser systems engineering, Glasses, Laser applications

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Proceedings Article | 24 April 1998 Paper

High-resolution real-time 3D imaging using time-gated photorefractive holography

Mary Tziraki, Richard Jones, Duncan Parsons-Karavassilis, Paul French, K. Kwolek, David Nolte, Michael Melloch

Proceedings Volume 3251, (1998) https://doi.org/10.1117/12.306042

KEYWORDS: Holography, 3D image reconstruction, Scattering, Holograms, Imaging systems, Video, 3D image processing, Tissues, Light scattering, Scattering media

Read Abstract +

Proceedings Article | 16 April 1998 Paper

Two-dimensional fluorescence lifetime imaging for in-vitro and in-vivo application

Paul French, Mark Dayel, Keith Dowling, Sam Hyde, M. Lever, P. Vourdas, Anthony Dymoke-Bradshaw, Jonathan Hares

Proceedings Volume 3250, (1998) https://doi.org/10.1117/12.305374

KEYWORDS: Fluorescence lifetime imaging, Luminescence, Picosecond phenomena, Tissues, Imaging systems, Tissue optics, Bioalcohols, Collagen, Sensors, In vitro testing

Read Abstract +

Proceedings Article | 13 January 1998 Paper

Time-gated holographic imaging using photorefractive media

Mary Tziraki, Richard Jones, Nicholas Barry, Sam Hyde, Paul French, K. Kwolek, David Nolte, Michael Melloch

Proceedings Volume 3196, (1998) https://doi.org/10.1117/12.297940

KEYWORDS: Holography, Scattering, Coherence (optics), Ultrafast phenomena, 3D image processing, Ferroelectric materials, Real time imaging, Quantum wells

Read Abstract +

Proceedings Article | 13 January 1998 Paper

High-resolution whole field fluorescence lifetime imaging of fluorophore distribution and environment

Mark Dayel, Keith Dowling, Sam Hyde, Christopher Dainty, Paul French, P. Vourdas, M. Lever, Anthony Dymoke-Bradshaw, Jonathan Hares, Paul Kellett

Proceedings Volume 3196, (1998) https://doi.org/10.1117/12.297925

KEYWORDS: Fluorescence lifetime imaging, Image intensifiers, Picosecond phenomena, Environmental sensing, Luminescence, Temporal resolution, Imaging systems, Proteins, Tissues

Read Abstract +

Proceedings Article | 1 November 1997 Paper

Novel ultrafast tunable solid state lasers for real-world applications including medical imaging

Nicholas Barry, Christopher Dainty, Keith Dowling, Paul French, Sam Hyde, Richard Jones, Richard Mellish, J. Sutherland, J. Taylor, Y. Tong, Bruce Chai, Carel van den Poel, Adriaan Valster

Proceedings Volume 3176, (1997) https://doi.org/10.1117/12.293466

Read Abstract +

Proceedings Article | 22 May 1997 Paper

Time-gated holographic imaging using photorefractive multiple quantum well devices

Richard Jones, Nicholas Barry, Sam Hyde, Christopher Dainty, Paul French, K. Kwolek, David Nolte, Michael Melloch

Proceedings Volume 2981, (1997) https://doi.org/10.1117/12.274306

KEYWORDS: Holography, Light scattering, Holograms, Scattering, Imaging systems, 3D image reconstruction, Quantum wells, Image resolution, Absorption, Scattering media

Read Abstract +

Proceedings Article | 7 May 1997 Paper

Two-dimensional fluorescence-lifetime imaging using a 5-kHz/110-ps gated image intensifier

Keith Dowling, Sam Hyde, Nicholas Barry, Christopher Dainty, Paul French, Alun Hughes, M. Lever, Anthony Dymoke-Bradshaw, Jonathan Hares, Paul Kellett

Proceedings Volume 2980, (1997) https://doi.org/10.1117/12.273522

KEYWORDS: Luminescence, Fluorescence lifetime imaging, Picosecond phenomena, Image intensifiers, Imaging systems, Tissues, Temporal resolution, Environmental sensing, Proteins, Tissue optics

Read Abstract +

Proceedings Article | 26 November 1996 Paper

Depth-resolved holography using photorefractive media

Nicholas Barry, Richard Jones, Sam Hyde, Christopher Dainty, Paul French, K. Kwoleck, David Nolte, Michael Melloch

Proceedings Volume 2928, (1996) https://doi.org/10.1117/12.259971

KEYWORDS: Scattering, Holograms, Light scattering, Holography, Crystals, Laser scattering, 3D image reconstruction, Diffraction gratings, Coherence (optics), Spatial filters

Read Abstract +

Proceedings Article | 15 January 1996 Paper

Time-gated holographic imaging using photorefractive media

Richard Jones, Nicholas Barry, Sam Hyde, Keith Dowling, Christopher Dainty, Paul French, Barry Wechsler, Marvin Klein

Proceedings Volume 2628, (1996) https://doi.org/10.1117/12.229987

KEYWORDS: Crystals, Holography, Scattering, Light scattering, Holograms, Image resolution, Laser crystals, Laser scattering, Imaging systems, Spatial resolution

Read Abstract +

Proceedings Article | 30 May 1995 Paper

Time-gated holographic imaging using photorefractive materials

Sam Hyde, Nicholas Barry, Richard Jones, Christopher Dainty, Paul French, Barry Wechsler, Marvin Klein

Proceedings Volume 2389, (1995) https://doi.org/10.1117/12.210011

KEYWORDS: Holography, Holograms, Scattering, Light scattering, Crystals, Laser scattering, Image resolution, Laser crystals, 3D image processing, Coherence (optics)

Read Abstract +

Proceedings Article | 10 April 1995 Paper

All solid state femtosecond laser oscillators and amplifiers

Sam Hyde, Nicholas Barry, Richard Mellish, Paul French, J. Taylor, Carel van den Poel, Adriaan Valster

Proceedings Volume 2377, (1995) https://doi.org/10.1117/12.206427

KEYWORDS: Femtosecond phenomena, Semiconductor lasers, Oscillators, Optical amplifiers, Mode locking, Argon ion lasers, Diodes, Amplifiers, Solid state lasers, Laser systems engineering

Read Abstract +

Showing 5 of 57 publications

Conference Committee Involvement (58)

Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XXIII

27 January 2025 | San Francisco, California, United States

Single Molecule Spectroscopy and Superresolution Imaging XVIII

25 January 2025 | San Francisco, California, United States

Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XXII

29 January 2024 | San Francisco, California, United States

Single Molecule Spectroscopy and Superresolution Imaging XVII

27 January 2024 | San Francisco, California, United States

Advances in Microscopic Imaging

28 June 2023 | Munich, Germany

Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XXI

30 January 2023 | San Francisco, California, United States

Single Molecule Spectroscopy and Superresolution Imaging XVI

28 January 2023 | San Francisco, California, United States

Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XX

24 January 2022 | San Francisco, California, United States

Single Molecule Spectroscopy and Superresolution Imaging XV

22 January 2022 | San Francisco, California, United States

Advances in Microscopic Imaging

20 June 2021 | Online Only, Germany

Single Molecule Spectroscopy and Superresolution Imaging XIV

6 March 2021 | Online Only, California, United States

Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XIX

6 March 2021 | Online Only, California, United States

Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVIII

3 February 2020 | San Francisco, California, United States

Single Molecule Spectroscopy and Superresolution Imaging XIII

1 February 2020 | San Francisco, California, United States

Advances in Microscopic Imaging

26 June 2019 | Munich, Germany

Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVII

4 February 2019 | San Francisco, California, United States

Multiphoton Microscopy in the Biomedical Sciences XIX

3 February 2019 | San Francisco, California, United States

Single Molecule Spectroscopy and Superresolution Imaging XII

2 February 2019 | San Francisco, California, United States

Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVI

29 January 2018 | San Francisco, California, United States

Multiphoton Microscopy in the Biomedical Sciences XVIII

28 January 2018 | San Francisco, California, United States

Single Molecule Spectroscopy and Superresolution Imaging XI

27 January 2018 | San Francisco, California, United States

Advances in Microscopic Imaging

25 June 2017 | Munich, Germany

Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XV

30 January 2017 | San Francisco, California, United States

Multiphoton Microscopy in the Biomedical Sciences XVII

29 January 2017 | San Francisco, California, United States

Single Molecule Spectroscopy and Superresolution Imaging X

28 January 2017 | San Francisco, California, United States

SPIE BioPhotonics Australasia

18 October 2016 | Adelaide, Australia

Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IX

15 February 2016 | San Francisco, California, United States

Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications VIII

15 February 2016 | San Francisco, California, United States

Multiphoton Microscopy in the Biomedical Sciences XVI

14 February 2016 | San Francisco, California, United States

Single Molecule Spectroscopy and Superresolution Imaging IX

13 February 2016 | San Francisco, California, United States

Advanced Microscopy Techniques IV

24 June 2015 | Munich, Germany

Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XIII

9 February 2015 | San Francisco, California, United States

Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications VII

9 February 2015 | San Francisco, California, United States

Multiphoton Microscopy in the Biomedical Sciences XV

8 February 2015 | San Francisco, California, United States

Single Molecule Spectroscopy and Superresolution Imaging VIII

7 February 2015 | San Francisco, California, United States

Biophotonics: Photonic Solutions for Better Health Care

14 April 2014 | Brussels, Belgium

Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XII

3 February 2014 | San Francisco, California, United States

Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications

3 February 2014 | San Francisco, California, United States

Multiphoton Microscopy in the Biomedical Sciences XIV

2 February 2014 | San Francisco, California, United States

Single Molecule Spectroscopy and Superresolution Imaging VII

1 February 2014 | San Francisco, California, United States

Advanced Microscopy Techniques III

12 May 2013 | Munich, Germany

Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications

4 February 2013 | San Francisco, California, United States

Multiphoton Microscopy in the Biomedical Sciences XIII

3 February 2013 | San Francisco, California, United States

Single Molecule Spectroscopy and Superresolution Imaging VI

2 February 2013 | San Francisco, California, United States

Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications

23 January 2012 | San Francisco, California, United States

Multiphoton Microscopy in the Biomedical Sciences XII

22 January 2012 | San Francisco, California, United States

Single Molecule Spectroscopy and Superresolution Imaging V

21 January 2012 | San Francisco, California, United States

Advanced Microscopy Techniques II

22 May 2011 | Munich, Germany

Multiphoton Microscopy in the Biomedical Sciences XI

23 January 2011 | San Francisco, California, United States

Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications

25 January 2010 | San Francisco, California, United States

Multiphoton Microscopy in the Biomedical Sciences X

24 January 2010 | San Francisco, California, United States

Single Molecule Spectroscopy and Imaging III

23 January 2010 | San Francisco, California, United States

Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications

26 January 2009 | San Jose, California, United States

Multiphoton Microscopy in the Biomedical Sciences IX

25 January 2009 | San Jose, California, United States

Single Molecule Spectroscopy and Imaging II

24 January 2009 | San Jose, California, United States

Biophotonics 2007: Optics in Life Science

18 June 2007 | Munich, Germany

Coherence Domain Optical Methods and Optical Coherence Tomography in Biomedicine VIII

26 January 2004 | San Jose, CA, United States

Coherence Domain Optical Methods and Optical Coherence Tomography in Biomedicine VII

27 January 2003 | San Jose, CA, United States

Showing 5 of 58 Conference Committees

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