The materials of experimental testing of the Stokes-polarimetry method using a reference laser wave are presented. The results of layer-by-layer measurement of coordinate distributions of the magnitude of the ellipticity of the polarization of laser radiation converted by polycrystalline films of biological fluids are presented. In the framework of the statistical and cross-correlation approaches, the values and ranges of changes in the statistical and correlation moments of the 1st to 4th orders of magnitude characterizing the distribution of the ellipticity of the polarization of laser radiation converted by polycrystalline networks in different phase sections are determined.
The results of the study of the relationships between 3D divisions of the optical anisotropy parameters of polycrystalline networks of films of biological fluids of various biochemical composition and layer-by-layer phase cross sections of volume distributions of the magnitude and phase parameters of the "two-point" Stokes vector of a microscopic image are presented. In the framework of the statistical approach using scale-selective wavelet analysis, the values and ranges of statistical 1st-4th order changes are defined, which characterize: • distribution of the values of the modulus and phase of the parameters of the Stokes vector in various phase sections of the object field; • a set of values of the amplitudes of the wavelet coefficients for various scales of the geometric dimensions of the module maps and the phase of the degree of correlation of the parameters of the Stokes vector (DCS).
A model of weak phase fluctuations of polycrystalline films of biological fluids is proposed. A correlation approach has been used to describe the polarization manifestations of the linear and circular birefringence of biological planar polycrystalline networks. Algorithms of polarization experimental measurement of the module (orientation map) and phase (phase map) of a set of "two-point" parameters of the Stokes vector are determined. The sets of orientation and phase maps of polycrystalline films of bile and blood are studied experimentally. The diagnostic possibilities of statistical analysis of the module and phase distributions of the "two-point" parameters of the Stokes vector of polarization-inhomogeneous images are considered. The magnitudes and ranges of changes in the set of statistical moments of the 1st and 4th orders that characterize the orientation and phase maps of polycrystalline films of bile and blood are found. The sensitivity, specificity and balanced accuracy of the method of polarization-correlation mapping in the diagnosis of early stage of cholelithiasis, as well as differentiation of the degree of blood losses, were determined.
Presented research materials: • coordinate maps of the degree of depolarization (CMD) of polycrystalline structures of histological sections of parenchymal (spleen) biological tissues of the deceased with different levels of blood loss; • magnitudes and ranges of changes in the statistical moments of the 1st - 4th orders characterizing the distribution of the magnitude of the CMD of biological organs (spleen, kidney) of the deceased with varying degrees of blood loss; • efficiency and accuracy of determining the degree of blood loss of the deceased by the method of diffuse Mueller-matrix polarimetry of polycrystalline structures of histological sections of parenchymal tissues of the deceased with different levels of blood loss.
This paper contains structural and logical scheme of the research; theoretical information about the set of azimuthally invariant Mueller-matrix elements and their combinations; The work is aimed at the development of a set of techniques that form a new method of azimuthally invariant differential polarimetry of partially-depolarizing optically anisotropic biological layers. This method is based on the determination and diagnostic use of a set of physical relationships between the distributions of azimuthally invariant polarization parameters characterizing the optical anisotropy of partially depolarizing layers of biological tissues, and the distributions of the parameters of linear and circular birefringence of such objects.
This report contains the results of approbations of the polarization correlometry method (PCM) – statistical mapping of biological tissues fractal structure (myocardium and brain - “fibrillar optically anisotropic networks” and the wall of the rectum - “island optically anisotropic structures”) and liquids (polycrystalline films of synovial fluid - a superposition of “structured and island networks of biological crystals").
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