We have recently developed a novel, cost-effective, versatile, and practical frequency-domain (FD) FLIM implementation capable of simultaneous multi-wavelength excitation, simultaneous multispectral detection, and sub-nanosecond to nanosecond fluorescence lifetime estimation. This novel technology has been adopted to implement an FD-FLIM endoscopy system for label-free metabolic imaging of epithelial tissues. The FD-FLIM endoscopy system is capable of simultaneous excitation at two wavelengths (375-nm for NADH, 445-nm for FAD). Preliminary results indicate that established metabolic autofluorescence biomarkers of epithelial pre-malignancy and malignancy can be clinically imaged with this novel FD-FLIM endoscopy technology. Future work will focus on exploring clinical applications of label-free metabolic imaging using this novel FD-FLIM endoscopy technology.
We report a new approach for label-free metabolic imaging of oral lesions using a novel fluorescence lifetime imaging endoscopy (FLIMEnd) system. The FLIMEnd system is capable of simultaneous dual-wavelength excitation and fluorescence detection at four emission spectral bands. Preliminary results indicate that established metabolic autofluorescence biomarkers of oral pre-malignancy and malignancy can be clinically imaged with this novel FLIMEnd technology. Future work will focus on identifying both established and novel autofluorescence biomarkers of oral pre-cancer and cancer that can be used to develop machine-learning driven FLIMEnd systems for early detection of oral cancer.
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