PicoQuant's rapidFLIMHiRes approach redefines Fluorescence Lifetime Imaging (FLIM) by combining fast data acquisition with n outstanding time resolution. This method is the ideal tool for ultra fast FLIM acquisiton with up to several frames per second and reliable, quantitative analysis for a broad range of applications such as: imaging of dynamic processes (e.g., protein interaction, chemical reaction, or ion flux), highly mobile species (e.g., mobility of cell organelles or particles, cell migration), high throughput FLIM screening, and investigating FRET dynamics. Recent hardware and software improvements allow for increased data acquisition speeds without sacrifices in lifetime resolution. rapidFLIMHiRes is available for PicoQuants's LSM Upgrade Kits and as a special configuration for the time-resolved fluorescence micrsocope MicroTime 200.
Fluorescence Lifetime Imaging (FLIM) is an essential tool in Life Sciences, but up to now users had to chose between high timing precision or fast data acquisition when using Time-Correlated Single Photon Counting (TCSPC) electronics. Our approach, named rapidFLIMHiRes, allows recording several FLIM images per second with a temporal resolution of 10 ps. The method combines advances in fast scanning, hybrid photomultiplier detectors, TCSPC modules, and correction algorithms to reduce decay curve distortions. Thus fast processes can be observed with the high optical and temporal resolution achievable in confocal microscopy at a rate of several frames per second.
Fluorescence Lifetime Imaging (FLIM) is an essential tool in Life Sciences, but up to now users had to chose between high timing precision or fast data acquisition when using Time-Correlated Single Photon Counting (TCSPC) electronics. Our approach, named rapidFLIMHiRes, allows recording several FLIM images per second with a temporal resolution of 10 ps. The method combines advances in fast scanning, hybrid photomultiplier detectors, TCSPC modules, and correction algorithms to reduce decay curve distortions. Thus fast processes can be observed with the high optical and temporal resolution achievable in confocal microscopy at a rate of several frames per second.
Access to the requested content is limited to institutions that have purchased or subscribe to SPIE eBooks.
You are receiving this notice because your organization may not have SPIE eBooks access.*
*Shibboleth/Open Athens users─please
sign in
to access your institution's subscriptions.
To obtain this item, you may purchase the complete book in print or electronic format on
SPIE.org.
INSTITUTIONAL Select your institution to access the SPIE Digital Library.
PERSONAL Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.