Unspecified Diana Boraschi Profile

Diana Boraschi

at Istituto di Biochimica e Biologia Cellulare

SPIE Involvement:

Author

Publications (1)

This will count as one of your downloads.

You will have access to both the presentation and article (if available).

DOWNLOAD NOW

This content is available for download via your institution's subscription. To access this item, please sign in to your personal account.

Email or Username Forgot your username?

Password Forgot your password?

Show

Keep me signed in

No SPIE account? Create an account

Proceedings Article | 20 June 2021 Presentation + Paper

SERS-based nanotoxicology assessment of gold nanoparticles

Alessandro Verde, Maria Mangini, Stefano Managò, Diana Boraschi, Paola Italiani, Anna Chiara De Luca

Proceedings Volume 11786, 117861G (2021) https://doi.org/10.1117/12.2593515

Read Abstract +

In the last few years, gold nanoparticles (AuNPs) have been widely used in biomedical applications; unfortunately, in some cases, they induce toxic effects on biological systems. Among these, the activation of the innate immune system (inflammatory response) is considered a central issue for assessing health risks. Although the origin of nanotoxicity is not well known, the cause could be associated with the presence of contaminants on nanoparticles surface, such as bacterial endotoxin. The bacterial endotoxin, also known as Lipopolysaccharide (LPS), is the main component of gram-negative bacteria cell walls and is considered one of the major contaminants in the environment. Therefore, AuNPs can be easily contaminated during the synthesis process or their manipulation, and nanotoxicological assessment has to be performed before using AuNPs for biomedical purposes. Traditional assays for LPS detection are not always effective because AuNPs could interfere with other components and alter the final readout. Surface-enhanced Raman spectroscopy (SERS) is a powerful tool for the analysis of molecules on metallic NPs, allowing to detect low concentration LPS with excellent sensitivity and reproducibility. In this work, we demonstrate that SERS technique can detect low amount of LPS (fg) on AuNPs (50 nm); moreover, we studied the internalization of bare and LPS-coated AuNPs in human monocyte-derived macrophages, by Raman Imaging, and their inflammatory effect by in vitro evaluation of pro and anti-inflammatory cytokine production.

My Library

You currently do not have any folders to save your paper to! Create a new folder below.

Folder Name

Folder Description

View contact details

UPDATE YOUR PROFILE

Is this your profile? Update it now.

Sign into your SPIE.org account

Don’t have a profile and want one?

Create an account on SPIE.org

Access to the requested content is limited to institutions that have purchased or subscribe to SPIE eBooks. You are receiving this notice because your organization may not have SPIE eBooks access.*

*Shibboleth/Open Athens users─please sign in to access your institution's subscriptions.

To obtain this item, you may purchase the complete book in print or electronic format on SPIE.org.

ORGANIZATIONAL
Sign in with credentials provided by your organization.

Organizational Username

Organizational Password

Show/Hide Password

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members:

Non-members: ADD TO CART

Keywords/Phrases

Search In:

Publication Years