Total internal reflection fluorescence (TIRF) is a popular type of illumination used in fluorescence microscopy for surface imaging of biospecimens; however, the commonly used objective-based TIRF illumination can induce artifacts that degrade the image. We previously demonstrated a fiber bundle that reshaped an input beam to achieve artifact-free TIRF illumination with coherent or incoherent light sources. Here we demonstrate an improved design using a photonic lantern, which will increase the power throughput by reducing coupling losses at the input facet. The improved power efficiency will facilitate new capabilities such as super-resolution imaging.
Photonic lanterns are being evaluated as a component of a scalable photon counting real-time optical ground receiver for space-to-ground photon-starved communication applications. The function of the lantern as a component of a receiver is to efficiently couple and deliver light from the atmospherically distorted focal spot formed behind a telescope to multiple small-core fiber-coupled single-element super-conducting nanowire detectors. This architecture solution is being compared to a multimode fiber coupled to a multi-element detector array. This paper presents a set of measurements that begins this comparison. This first set of measurements are a comparison of the throughput coupling loss at emulated atmospheric conditions for the case of a 60 cm diameter telescope receiving light from a low earth orbit satellite. The atmospheric conditions are numerically simulated at a range of turbulence levels using a beam propagation method and are physically emulated with a spatial light modulator. The results show that for the same number of output legs as the single-mode fiber lantern, the few-mode fiber lantern increases the power throughput up to 3.92 dB at the worst emulated atmospheric conditions tested of D/r0=8.6. Furthermore, the coupling loss of the few-mode fiber lantern approaches the capability of a 30 micron graded index multimode fiber chosen for coupling to a 16 element detector array.
We demonstrated flat-field illumination (FFI) for multi-color wide-field fluorescence microscopy using a refractionbased beam shaping system. The non-homogeneous illumination of a Gaussian intensity profile makes quantitative analysis in laser-assisted wide-field fluorescence microscopy very difficult. As contrasted with other approaches, our method is applicable to TIRF illumination, which effectively rejects background fluorescence.
Our beam shaping device is extremely tolerant to variations in size of the incoming laser beam by accepting ± 10% variation, while being achromatic as well. This behavior originates from the well-balanced mapping of the incoming rays to the intended flattop beam profile in combination with a sophisticated material choice, which decreases the sensitivity to input beam diameter. The homogenous illumination profile of FFI will enable quantitative single-molecule analysis based on intensity information. This has powerful implications when combined with a pull-down assay, which can probe the oligomerization state of endogenous proteins. When combined with one-to-one fluorophore labeling, the stoichiometry of proteins related to neurodegenerative diseases could be readily determined by intensity distribution analysis, which is critical to not only diagnosing but also understanding the pathogenesis of these complex disorders that are particularly difficult to analyze.
An additional application of FFI is high quality super-resolution imaging with a uniform spatial resolution over a large FOV, where the full power of the excitation beam could be utilized. A new optical design approach based on refractive freeform surfaces generating a square-shaped beam instead of a round one will be presented, which would yield greater illumination efficiency.
We demonstrated flat-field illumination (FFI) for multi-color wide-field fluorescence microscopy using a refractionbased beam shaping system. The non-homogeneous illumination of a Gaussian intensity profile makes quantitative analysis in laser-assisted wide-field fluorescence microscopy very difficult. As contrasted with other approaches, our method is applicable to TIRF illumination, which effectively rejects background fluorescence. Our beam shaping device is extremely tolerant to variations in size of the incoming laser beam by accepting ± 10% variation, while being achromatic as well. This behavior originates from the well-balanced mapping of the incoming rays to the intended flattop beam profile in combination with a sophisticated material choice, which decreases the sensitivity to input beam diameter. The homogenous illumination profile of FFI will enable quantitative single-molecule analysis based on intensity information. This has powerful implications when combined with a pull-down assay, which can probe the oligomerization state of endogenous proteins. When combined with one-to-one fluorophore labeling, the stoichiometry of proteins related to neurodegenerative diseases could be readily determined by intensity distribution analysis, which is critical to not only diagnosing but also understanding the pathogenesis of these complex disorders that are particularly difficult to analyze. An additional application of FFI is high quality super-resolution imaging with a uniform spatial resolution over a large FOV, where the full power of the excitation beam could be utilized. A new optical design approach based on refractive freeform surfaces generating a square-shaped beam instead of a round one will be presented, which would yield greater illumination efficiency.
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