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5 April 2016 Bioluminescence imaging in live cells and animals
Jack K. Tung, Ken Berglund, Claire-Anne Gutekunst, Ute Hochgeschwender, Robert E. Gross
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Neurophotonics, Vol. 3, Issue 2, 025001 (April 2016). https://doi.org/10.1117/1.NPh.3.2.025001
Abstract
The use of bioluminescent reporters in neuroscience research continues to grow at a rapid pace as their applications and unique advantages over conventional fluorescent reporters become more appreciated. Here, we describe practical methods and principles for detecting and imaging bioluminescence from live cells and animals. We systematically tested various components of our conventional fluorescence microscope to optimize it for long-term bioluminescence imaging. High-resolution bioluminescence images from live neurons were obtained with our microscope setup, which could be continuously captured for several hours with no signs of phototoxicity. Bioluminescence from the mouse brain was also imaged noninvasively through the intact skull with a conventional luminescence imager. These methods demonstrate how bioluminescence can be routinely detected and measured from live cells and animals in a cost-effective way with common reagents and equipment.
CC BY: © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.
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Jack K. Tung, Ken Berglund, Claire-Anne Gutekunst, Ute Hochgeschwender, and Robert E. Gross "Bioluminescence imaging in live cells and animals," Neurophotonics 3(2), 025001 (5 April 2016). https://doi.org/10.1117/1.NPh.3.2.025001
Published: 5 April 2016
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KEYWORDS
Bioluminescence
Cameras
Live cell imaging
Luminescence
In vivo imaging
Proteins
Microscopes
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