Toward multi-focal spot remote focusing two-photon microscopy for high speed imaging

Bei Li; Alexander D. Corbett; Ee Chong; Edward Mann; Tony Wilson; Martin J. Booth; Gil Bub

doi:10.1117/12.2251214

17 February 2017 Toward multi-focal spot remote focusing two-photon microscopy for high speed imaging

Bei Li, Alexander D. Corbett, Ee Chong, Edward Mann, Tony Wilson, Martin J. Booth, Gil Bub

Proceedings Volume 10070, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXIV; 1007005 (2017) https://doi.org/10.1117/12.2251214
Event: SPIE BiOS, 2017, San Francisco, California, United States

Abstract

Optical sectioning techniques using two-photon excitation of fluorescent indicators are central to diverse imaging applications. The limitations of the technique are low speed and undesirable specimen agitation. In our design, high-speed axial scanning is carried out by moving a reference objective to axially displace the focal spot without introducing significant spherical aberration and any agitation of the specimen. Further, the system is configured to allow switching between single spot and multiple focal spot remote focusing to allow either high dynamic range or high speed imaging.

Citation Download Citation

Bei Li, Alexander D. Corbett, Ee Chong, Edward Mann, Tony Wilson, Martin J. Booth, and Gil Bub "Toward multi-focal spot remote focusing two-photon microscopy for high speed imaging", Proc. SPIE 10070, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXIV, 1007005 (17 February 2017); https://doi.org/10.1117/12.2251214

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
5 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Objectives

Luminescence

High speed imaging

Signal detection

Electron multiplying charge coupled devices

Microscopes

Mirrors

Show All Keywords

Keywords/Phrases

Search In:

Publication Years