In vivo near-infrared dual-axis confocal microendoscopy in the human lower gastrointestinal tract

Wibool Piyawattanametha; Hyejun Ra; Kevin E. Loewke; Michael J. Mandella; Christopher H. Contag; Zhen Qiu; Thomas D. Wang; Shai Friedland; Jonathan T. C. Liu; Gordon S. Kino; Olav Solgaard

doi:10.1117/1.JBO.17.2.021102

2 March 2012 In vivo near-infrared dual-axis confocal microendoscopy in the human lower gastrointestinal tract

Wibool Piyawattanametha, Hyejun Ra, Kevin E. Loewke, Michael J. Mandella, Christopher H. Contag, Zhen Qiu, Thomas D. Wang, Shai Friedland, Jonathan T. C. Liu, Gordon S. Kino, Olav Solgaard

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Journal of Biomedical Optics, Vol. 17, Issue 2, 021102 (March 2012). https://doi.org/10.1117/1.JBO.17.2.021102

Abstract

Near-infrared confocal microendoscopy is a promising technique for deep in vivo imaging of tissues and can generate high-resolution cross-sectional images at the micron-scale. We demonstrate the use of a dual-axis confocal (DAC) near-infrared fluorescence microendoscope with a 5.5-mm outer diameter for obtaining clinical images of human colorectal mucosa. High-speed two-dimensional en face scanning was achieved through a microelectromechanical systems (MEMS) scanner while a micromotor was used for adjusting the axial focus. In vivo images of human patients are collected at 5 frames/sec with a field of view of 362×212 μm² and a maximum imaging depth of 140 μm. During routine endoscopy, indocyanine green (ICG) was topically applied a nonspecific optical contrasting agent to regions of the human colon. The DAC microendoscope was then used to obtain microanatomic images of the mucosa by detecting near-infrared fluorescence from ICG. These results suggest that DAC microendoscopy may have utility for visualizing the anatomical and, perhaps, functional changes associated with colorectal pathology for the early detection of colorectal cancer.

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Wibool Piyawattanametha, Hyejun Ra, Kevin E. Loewke, Michael J. Mandella, Christopher H. Contag, Zhen Qiu, Thomas D. Wang, Shai Friedland, Jonathan T. C. Liu, Gordon S. Kino, and Olav Solgaard "In vivo near-infrared dual-axis confocal microendoscopy in the human lower gastrointestinal tract," Journal of Biomedical Optics 17(2), 021102 (2 March 2012). https://doi.org/10.1117/1.JBO.17.2.021102

Published: 2 March 2012

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