Mechanobiology of T cells during early activation as monitored through optical trapping microrheology

Elizabeth L. Holliday; Daniel J. Masi; Katarzyna J. Zienkiewicz; Aoi Akitsu; Ellis L. Reinherz; Matthew J. Lang

doi:10.1117/12.3029869

3 October 2024 Mechanobiology of T cells during early activation as monitored through optical trapping microrheology

Elizabeth L. Holliday, Daniel J. Masi, Katarzyna J. Zienkiewicz, Aoi Akitsu, Ellis L. Reinherz, Matthew J. Lang

Proceedings Volume PC13112, Optical Trapping and Optical Micromanipulation XXI; PC1311202 (2024) https://doi.org/10.1117/12.3029869
Event: Nanoscience + Engineering, 2024, San Diego, California, United States

Abstract

The mechanosensitive nature of the TCR-pMHC interaction in T cells has been studied via optical tweezer experiments. From these studies, populations of force responsive T cells (digital cells) that recover activation potential in sparse pMHC environments have been discovered. Through a series of optical tweezer experiments, the distinctions between the digital population and their counterpart T cells will be examined. These include triggering ability at varying pMHC copy numbers and the stiffness changes in the T cell membrane as measured through microrheology.

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Citation Download Citation

Elizabeth L. Holliday, Daniel J. Masi, Katarzyna J. Zienkiewicz, Aoi Akitsu, Ellis L. Reinherz, and Matthew J. Lang "Mechanobiology of T cells during early activation as monitored through optical trapping microrheology", Proc. SPIE PC13112, Optical Trapping and Optical Micromanipulation XXI, PC1311202 (3 October 2024); https://doi.org/10.1117/12.3029869

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