Combined optical trapping and fluorescence for single T cell activation profiling

Daniel Masi; Elizabeth Holliday; Katarzyna Zienkiewicz; Aoi Akitsu; Ellis Reinherz; Matthew Lang

doi:10.1117/12.3029874

3 October 2024 Combined optical trapping and fluorescence for single T cell activation profiling

Daniel Masi, Elizabeth Holliday, Katarzyna Zienkiewicz, Aoi Akitsu, Ellis Reinherz, Matthew Lang

Proceedings Volume PC13112, Optical Trapping and Optical Micromanipulation XXI; PC1311219 (2024) https://doi.org/10.1117/12.3029874
Event: Nanoscience + Engineering, 2024, San Diego, California, United States

Abstract

T cell clones can exhibit varying potencies towards their cognate peptide major histocompatibility complex molecule (pMHC), which may not be seen in traditional bulk assays. Performing single molecule and single cell experiments, including profiling through Single Cell Activation Requirements allow for further discrimination and selection of the highest quality clones. Using these techniques will help reveal how T cells function and advance screening methods for selecting the best T cells to use therapeutically.

Conference Presentation

Citation Download Citation

Daniel Masi, Elizabeth Holliday, Katarzyna Zienkiewicz, Aoi Akitsu, Ellis Reinherz, and Matthew Lang "Combined optical trapping and fluorescence for single T cell activation profiling", Proc. SPIE PC13112, Optical Trapping and Optical Micromanipulation XXI, PC1311219 (3 October 2024); https://doi.org/10.1117/12.3029874

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