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This study is focused on the determination of the real and imaginary parts of the complex refractive index of
oxyhemoglobin and deoxyhemoglobin solutions (1.6 g/l) at different concentrations of glucose (from 0 to 1000 mg/dl
with a step of 100 mg/dl) from a few hours to over one week of incubation. The real part was measured using Abbe
refractometer. Theoretical values of real part of refractive index were calculated supposing non-interacting hemoglobin
and glucose molecules. The imaginary part was determined using transmittance measurements at the wavelength range
from 500 nm to 1000 nm. It is shown that the real and imaginary parts of the complex refractive index measurements
have a potential for the evaluation of glycated hemoglobin amount.
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Ekaterina N. Lazareva, Valery V. Tuchin, "Monitoring of hemoglobin glycation using spectral and refraction measurements," Proc. SPIE 6534, Fifth International Conference on Photonics and Imaging in Biology and Medicine, 653426 (1 May 2007); https://doi.org/10.1117/12.741636