Detection of cell metabolism via wavelength- and time-resolved intracellular autofluorescence

Yicong Wu; Wei Zheng; Jianan Y. Qu

doi:10.1117/12.702459

6 February 2007 Detection of cell metabolism via wavelength- and time-resolved intracellular autofluorescence

Yicong Wu, Wei Zheng, Jianan Y. Qu

Proceedings Volume 6430, Advanced Biomedical and Clinical Diagnostic Systems V; 64300A (2007) https://doi.org/10.1117/12.702459
Event: SPIE BiOS, 2007, San Jose, California, United States

Abstract

A time-resolved confocal fluorescence spectroscopy system was instrumented utilizing the multi-channel time-correlated single photon counting (TCSPC) technique. The system provided a unique approach to investigate the relationship between wavelength- and time-resolved cell autofluorescence and cellular metabolic status. The experiments were carried out on monolayered cell cultures including normal and cancer ectocervical cells. With UV excitation at 365 nm, the decay of cellular fluorescence can be well described by a dual-exponential function, consisting of a short lifetime component ((tau)₁~ 0.40 - 0.47 ns) and a long lifetime component (((tau)₂ ~ 3.3 - 4.0 ns). By analyzing the decay-associated spectra of the short and long lifetime components, we found that the long lifetime component carried the information of protein-bound NADH and short lifetime component was mainly determined by free NADH with certain interference from bound NADH. Moreover, it was found that the ratio of the amplitudes of two lifetime components, dominated by free/bound NADH, was sensitive to cell metabolism. Overall, this study demonstrated that wavelength- and timeresolved autofluorescence can be potentially used as an important contrast mechanism to detect epithelial pre-cancer.

Citation Download Citation

Yicong Wu, Wei Zheng, and Jianan Y. Qu "Detection of cell metabolism via wavelength- and time-resolved intracellular autofluorescence", Proc. SPIE 6430, Advanced Biomedical and Clinical Diagnostic Systems V, 64300A (6 February 2007); https://doi.org/10.1117/12.702459

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available