Combined time- and depth-resolved autofluorescence spectroscopy for tissue diagnosis

Yicong Wu; Jianan Y. Qu

doi:10.1117/12.651391

25 February 2006 Combined time- and depth-resolved autofluorescence spectroscopy for tissue diagnosis

Yicong Wu, Jianan Y. Qu

Proceedings Volume 6080, Advanced Biomedical and Clinical Diagnostic Systems IV; 608018 (2006) https://doi.org/10.1117/12.651391
Event: SPIE BiOS, 2006, San Jose, California, United States

Abstract

A fluorescence spectroscopy system combining depth- and time-resolved measurements is developed to investigate the layered fluorescence temporal characteristics of epithelial tissue. It is found that esophageal tissue structure can be resolved well by means of the autofluorescence time-resolved decay process with 375-, 405- and 435- nm excitation. The decay of the autofluorescence signals can be accurately fitted with a dual-exponential function consisting of a short lifetime (0.4 ~ 0.6 ns) and a long lifetime (3 ~ 4 ns) components. The short lifetime component dominates the decay of normal epithelial fluorescence while the decay of the signals from keratinized epithelium and stroma are mainly determined by the long lifetime component. The ratio of the amplitudes of two components provides the information of fine structure of epithelial tissue. This study demonstrates that the combined depth- and time-resolved measurements can potentially provide accurate information for the diagnosis of tissue pathology.

Citation Download Citation

Yicong Wu and Jianan Y. Qu "Combined time- and depth-resolved autofluorescence spectroscopy for tissue diagnosis", Proc. SPIE 6080, Advanced Biomedical and Clinical Diagnostic Systems IV, 608018 (25 February 2006); https://doi.org/10.1117/12.651391

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