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Aging related biological mechanisms are often difficult to probe in situ without exogenous fluorophores. Here, we leverage label-free stimulated Raman scattering (SRS) microscopy to provide new insights into aging in C. elegans. We demonstrate multispectral SRS imaging of whole worms in vivo with quantitative chemical insights across different ages. We show that both lipid and protein synthesis and compartmentalization are associated with aging in worms. Moreover, we observe notable SRS image differences when worms are subjected to calorie restriction, suggesting a promising avenue towards understanding calorie restriction’s enhancing effects on longevity when coupled to proteomic and metabolomic analysis.
Bryce Manifold,Bowen Yang,Denis Titov, andAaron Streets
"Measuring chemical changes during aging in vivo with label-free stimulated Raman scattering microscopy", Proc. SPIE PC12855, Advanced Chemical Microscopy for Life Science and Translational Medicine 2024, PC128550C (13 March 2024); https://doi.org/10.1117/12.3003529
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Bryce Manifold, Bowen Yang, Denis Titov, Aaron Streets, "Measuring chemical changes during aging in vivo with label-free stimulated Raman scattering microscopy," Proc. SPIE PC12855, Advanced Chemical Microscopy for Life Science and Translational Medicine 2024, PC128550C (13 March 2024); https://doi.org/10.1117/12.3003529