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We present a reversible reaction-based Raman probe for live-cell imaging of Glutathione (GSH) by the hyperspectral stimulated Raman scattering microscopy. By introducing a cyano group as the Raman reporter and an alkyne group as the internal standard, we have designed, synthesized, and characterized a series of Raman-based probes for ratiometric imaging and quantification of GSH levels in living cells and organisms.
Dinghuan Deng,Jianwei Chen,Tao Chen,Jin Wang, andMeng C. Wang
"Quantitative imaging of glutathione using hyperspectral stimulated Raman scattering microscopy", Proc. SPIE PC11973, Advanced Chemical Microscopy for Life Science and Translational Medicine 2022, PC119730Z (2 March 2022); https://doi.org/10.1117/12.2610245
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Dinghuan Deng, Jianwei Chen, Tao Chen, Jin Wang, Meng C. Wang, "Quantitative imaging of glutathione using hyperspectral stimulated Raman scattering microscopy," Proc. SPIE PC11973, Advanced Chemical Microscopy for Life Science and Translational Medicine 2022, PC119730Z (2 March 2022); https://doi.org/10.1117/12.2610245