Dr. Janelle M. P. Pakan Profile

Dr. Janelle M. P. Pakan

at Otto-von-Guericke-Univ Magdeburg

SPIE Involvement:

Conference Program Committee | Author

Publications (2)

SPIE Journal Paper | 13 February 2024 Open Access

Advancing the path to in-vivo imaging in freely moving mice via multimode-multicore fiber based holographic endoscopy

Yang Du, Evelyn Dylda, Miroslav Stibůrek, André D Gomes, Sergey Turtaev, Janelle M. P. Pakan, Tomáš Čižmár

NPh, Vol. 11, Issue S1, S11506, (February 2024) https://doi.org/10.1117/12.10.1117/1.NPh.11.S1.S11506

KEYWORDS: In vivo imaging, Multicore fiber, Multimode fibers, Brain, Animals, Structured optical fibers, Neurons, Digital micromirror devices, Neurophotonics, Neuroimaging

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SignificanceHair-thin multimode optical fiber-based holographic endoscopes have gained considerable interest in modern neuroscience for their ability to achieve cellular and even subcellular resolution during in-vivo deep brain imaging. However, the application of multimode fibers in freely moving animals presents a persistent challenge as it is difficult to maintain optimal imaging performance while the fiber undergoes deformations.AimWe propose a fiber solution for challenging in-vivo applications with the capability of deep brain high spatial resolution imaging and neuronal activity monitoring in anesthetized as well as awake behaving mice.ApproachWe used our previously developed M3CF multimode-multicore fiber to record fluorescently labeled neurons in anesthetized mice. Our M3CF exhibits a cascaded refractive index structure, enabling two distinct regimes of light transport that imitate either a multimode or a multicore fiber. The M3CF has been specifically designed for use in the initial phase of an in-vivo experiment, allowing for the navigation of the endoscope’s distal end toward the targeted brain structure. The multicore regime enables the transfer of light to and from each individual neuron within the field of view. For chronic experiments in awake behaving mice, it is crucial to allow for disconnecting the fiber and the animal between experiments. Therefore, we provide here an effective solution and establish a protocol for reconnection of two segments of M3CF with hexagonally arranged corelets.ResultsWe successfully utilized the M3CF to image neurons in anaesthetized transgenic mice expressing enhanced green fluorescent protein. Additionally, we compared imaging results obtained with the M3CF with larger numerical aperture (NA) fibers in fixed whole-brain tissue.ConclusionsThis study focuses on addressing challenges and providing insights into the use of multimode-multicore fibers as imaging solutions for in-vivo applications. We suggest that the upcoming version of the M3CF increases the overall NA between the two cladding layers to allow for access to high resolution spatial imaging. As the NA increases in the multimode regime, the fiber diameter and ring structure must be reduced to minimize the computational burden and invasiveness.

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Proceedings Article | 4 March 2019 Presentation

In-vivo deep-brain imaging through a single fibre endoscope (Conference Presentation)

Sergey Turtaev, Ivo Leite, Tristan Altwegg-Boussac, Janelle M. Pakan, Nathalie Rochefort, Tomas Cizmar

Proceedings Volume 10886, 1088604 (2019) https://doi.org/10.1117/12.2510432

KEYWORDS: Endoscopes, In vivo imaging, Neuroscience, Tissues, Holography, Multimode fibers, Image resolution, Imaging systems, Visual process modeling, Mode conditioning cables

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Conference Committee Involvement (1)

Neurophotonics II

8 April 2024 | Strasbourg, France

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