Tracking of C. Reinhardtii flagellar disassembly following femtosecond laser ablation

Katelyn Lee; Hiro Ishikawa; Celina Jiang; Bethany Campbell; Elise Stuman; Jiayu Hu; Veronica Gomez; Wallace Marshall; Linda Shi

doi:10.1117/12.2677805

5 October 2023 Tracking of C. Reinhardtii flagellar disassembly following femtosecond laser ablation

Katelyn Lee, Hiro Ishikawa, Celina Jiang, Bethany Campbell, Elise Stuman, Jiayu Hu, Veronica Gomez, Wallace Marshall, Linda Shi

Author Affiliations +

Proceedings Volume 12649, Optical Trapping and Optical Micromanipulation XX; 126490T (2023) https://doi.org/10.1117/12.2677805
Event: SPIE Nanoscience + Engineering, 2023, San Diego, California, United States

Conference Poster

Abstract

Single-cell green algae (C. Reinhardtii) is a key model organism to study ciliogenesis. Cilia have important roles in sensory signaling pathways and in clearing the airways of mucus and dirt in multiple systems of the human body. As cilia are found on most eukaryotic cells, defects in ciliogenesis result in many symptoms and disorders. We are testing the hypothesis that when a flagellum is removed, the long flagellum shrinks because it is competing with the shorter regrowing flagellum. We used a 780-nm 200-fs laser to perform laser ablation to amputate one of two flagella on wildtype and mutant algae. Fla3 and Fla10 mutants were altered to inhibit the KAP kinesin motor that drives the intraflagellar transport (IFT) pathway. Impaired IFT pathways would demonstrate a lag in response to flagellar length equalization and a reduced disassembly rate. Quantified images following the long flagellum for 20 min post-ablation demonstrate a delayed disassembly rate in the Fla3 mutant compared to wildtype; Fla10 was inconclusive. Therefore, it was concluded that the proper function of KAP motor protein serves a significant role in length control of cilia. In the future, we will compare the assembly rates of flagellar regrowth for the wildtype and mutants.

(2023) Published by SPIE. Downloading of the abstract is permitted for personal use only.

Citation Download Citation

Katelyn Lee, Hiro Ishikawa, Celina Jiang, Bethany Campbell, Elise Stuman, Jiayu Hu, Veronica Gomez, Wallace Marshall, and Linda Shi "Tracking of C. Reinhardtii flagellar disassembly following femtosecond laser ablation", Proc. SPIE 12649, Optical Trapping and Optical Micromanipulation XX, 126490T (5 October 2023); https://doi.org/10.1117/12.2677805

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available