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5 March 2021 Characterization and quantification of necrotic regions and morphology in multicellular tumor spheroids using optical coherence tomography
Feng Yan, Gokhan Gunay, Trisha I. Valerio, Chen Wang, Jayla A. Wilson, Maegan M. Watson, Michael T. OConnell, Handan Acar, Qinggong Tang
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Proceedings Volume 11657, Biomedical Applications of Light Scattering XI; 116570H (2021) https://doi.org/10.1117/12.2578750
Event: SPIE BiOS, 2021, Online Only
Abstract
Three-dimensional (3D) cell culture models are developed as a promising platform to screen anticancer therapeutics and treatments. However, current imaging techniques cannot provide 3D structures of tumor spheroids in situ. In this study, we employed label-free and noninvasive optical coherence tomography (OCT) for imaging and quantifying the 3D structures of tumor spheroids. We imaged ovarian cancer spheroids with OVCAR-8 cell line over a period of 10 days with 5,000 and 50,000 initial cell numbers. We successfully reconstructed the 3D necrotic regions via label-free intrinsic scattering attenuation contrast and evaluated the effect of Cisplatin treatment on tumor spheroids.
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Feng Yan, Gokhan Gunay, Trisha I. Valerio, Chen Wang, Jayla A. Wilson, Maegan M. Watson, Michael T. OConnell, Handan Acar, and Qinggong Tang "Characterization and quantification of necrotic regions and morphology in multicellular tumor spheroids using optical coherence tomography", Proc. SPIE 11657, Biomedical Applications of Light Scattering XI, 116570H (5 March 2021); https://doi.org/10.1117/12.2578750
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KEYWORDS
Tumors
3D modeling
Optical coherence tomography
3D displays
Multiphoton microscopy
3D image processing
Luminescence
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