Instantaneous acquisition and analysis of intravital 3D fluorescence lifetime imaging microscopy

Scott S. Howard

doi:10.1117/12.2615412

2 March 2022 Instantaneous acquisition and analysis of intravital 3D fluorescence lifetime imaging microscopy

Scott S. Howard

Proceedings Volume PC11971, High-Speed Biomedical Imaging and Spectroscopy VII; PC119710H (2022) https://doi.org/10.1117/12.2615412
Event: SPIE BiOS, 2022, San Francisco, California, United States

Abstract

Fluorescence lifetime imaging microscopy (FLIM) adds an additional dimension to fluorescence microscopy by measuring the fluorophore interactions with the microenvironment in addition to all the benefits and power of fluorescence microscopy. Real-time FLIM, however, requires overcoming unique technical challenges to achieve similar imaging speeds as can be achieved through standard in vivo microscopy techniques. This talk will present an “InstantFLIM” system that achieves real-time (acquisition and processing), super-resolution, 3D in vivo multiphoton FLIM by overcoming these limitations. The system is demonstrated in intact-skull mouse and zebrafish brain imaging models, and 3D autofluorescence FLIM of highly scattering plant tissue.

Conference Presentation

Citation Download Citation

Scott S. Howard "Instantaneous acquisition and analysis of intravital 3D fluorescence lifetime imaging microscopy", Proc. SPIE PC11971, High-Speed Biomedical Imaging and Spectroscopy VII, PC119710H (2 March 2022); https://doi.org/10.1117/12.2615412

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