Use of single molecule techniques to investigate molecular mechanisms behind memory formation (Conference Presentation)

Sofia Vargas-Hernandez; Tyler Nelson; Yuya Nakatani; Ivana Hsyung; Margareth Freire; M. Neal Waxham; Anna-Karin Gustavsson

doi:10.1117/12.2650681

15 March 2023 Use of single molecule techniques to investigate molecular mechanisms behind memory formation (Conference Presentation)

Sofia Vargas-Hernandez, Tyler Nelson, Yuya Nakatani, Ivana Hsyung, Margareth Freire, M. Neal Waxham, Anna-Karin Gustavsson

Author Affiliations +

Proceedings Volume PC12386, Single Molecule Spectroscopy and Superresolution Imaging XVI; PC1238608 (2023) https://doi.org/10.1117/12.2650681
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

Dendritic spines are neuronal structures which play a key role in memory formation by strengthening synaptic pathways through actin cytoskeleton reorganization. The protein CaMKII is hypothesized to perform major structural functions in this rearrangement. Here, we use single-particle tracking and super-resolution imaging to extract information on the bindings dynamic and nanoscale architecture of reconstituted CaMKII-actin assemblies adhered to a coverslip. To improve resolution over the entire field of view, we use a homogeneously illuminated total internal reflection fluorescence scheme. Our method closes an experimental gap present in the field and provides relevant knowledge of actin/CaMKII interactions at the nanoscale.

Conference Presentation

Citation Download Citation

Sofia Vargas-Hernandez, Tyler Nelson, Yuya Nakatani, Ivana Hsyung, Margareth Freire, M. Neal Waxham, and Anna-Karin Gustavsson "Use of single molecule techniques to investigate molecular mechanisms behind memory formation (Conference Presentation)", Proc. SPIE PC12386, Single Molecule Spectroscopy and Superresolution Imaging XVI, PC1238608 (15 March 2023); https://doi.org/10.1117/12.2650681

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
PRESENTATION

WATCH
PRESENTATION SAVE TO MY LIBRARY

GET CITATION

KEYWORDS

Molecular mechanisms

Molecules

Network architectures

Dendrites

Luminescence

Neurons

Particles

Show All Keywords

Keywords/Phrases

Search In:

Publication Years