Dendritic spines are neuronal structures which play a key role in memory formation by strengthening synaptic pathways through actin cytoskeleton reorganization. The protein CaMKII is hypothesized to perform major structural functions in this rearrangement.
Here, we use single-particle tracking and super-resolution imaging to extract information on the bindings dynamic and nanoscale architecture of reconstituted CaMKII-actin assemblies adhered to a coverslip.
To improve resolution over the entire field of view, we use a homogeneously illuminated total internal reflection fluorescence scheme. Our method closes an experimental gap present in the field and provides relevant knowledge of actin/CaMKII interactions at the nanoscale.
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