The analysis of Hela cells are very dependent on the segmentation of cell images, but the difficulty of cell image segmentation comes from two aspects: cell itself and imaging technology. we present a method for automatic segmentation of Hela cell imaging in digital holography(DH) in this paper. By using a DH imaging system and a cell segmentation algorithm based on adaptive threshold segmentation and watershed algorithm, cells that adhere to each other or have irregular shapes in the image can be segmented.
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