Ms. Gail P. Sudlow Profile

Gail P. Sudlow

Research Assistant at Mallinckrodt Institute of Radiology

Area of Expertise:

animal imaging , fluorescence microscopy , histology , tissue culture

Publications (17)

Proceedings Article | 5 March 2021 Presentation

Presentation

Focal dynamic thermal imaging for label-free detection of cancer in preclinical models

Christine O'Brien, Hongyu Meng, Leonid Shmuylovich, Julia Carpenter, Praneeth Gogineni, Haini Zhang, Kevin Bishop, Suman Mondal, Gail Sudlow, Cheryl Bethea, Clyde Bethea, Samuel Achilefu

Proceedings Volume 11651, 116510R (2021) https://doi.org/10.1117/12.2583435

KEYWORDS: Cancer, Tissues, Thermography, Tumor growth modeling, Thermal modeling, Tumors, 3D modeling, Thermal energy technology, Systems modeling, Real time imaging

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Proceedings Article | 5 March 2021 Presentation

Presentation

Dual fluorescence guidance improves extent of brain tumor removal surgery

Haini Zhang, Suman Mondal, Dorota Grabowska, Matt Mixdorf, Gail Sudlow, Christine O'Brien, Julie Prior, Kexian Liang, Rui Tang, Samuel Achilefu

Proceedings Volume 11660, 1166009 (2021) https://doi.org/10.1117/12.2577287

KEYWORDS: Tumors, Surgery, Photodynamic therapy, Brain, Luminescence, Visible radiation, Tissue optics, Brain cancer, In vivo imaging, In vitro testing

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SPIE Journal Paper | 28 February 2020 Open Access

Open Access

Intracranial glioma xenograft model rapidly reestablishes blood–brain barrier integrity for longitudinal imaging of tumor progression using fluorescence molecular tomography and contrast agents

LeMoyne Habimana-Griffin, Dezhuang Ye, Julia Carpenter, Julie Prior, Gail Sudlow, Lynne Marsala, Matthew Mixdorf, Joshua Rubin, Hong Chen, Samuel Achilefu

JBO, Vol. 25, Issue 02, 026004, (February 2020) https://doi.org/10.1117/12.10.1117/1.JBO.25.2.026004

KEYWORDS: Tumors, Luminescence, Brain, Blood brain barrier, Neuroimaging, Near infrared, Tissues, Fluorescence tomography, Tomography, Bioluminescence

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Significance: The blood–brain barrier (BBB) is a major obstacle to detecting and treating brain tumors. Overcoming this challenge will facilitate the early and accurate detection of brain lesions and guide surgical resection of tumors. Aim: We generated an orthotopic brain tumor model that simulates the pathophysiology of gliomas at early stages; determine the BBB integrity and breakdown over the time course of tumor progression using generic and cancer-targeted near-infrared (NIR) fluorescent molecular probes. Approach: We developed an intracranial tumor xenograft model that rapidly reestablished BBB integrity and monitored tumor progression by bioluminescence imaging. Sham control mice were injected with phosphate-buffered saline only. Fluorescence molecular tomography (FMT) was used to quantify the uptake of tumor-targeted and passive NIR fluorescent imaging agents in orthotopic glioma (U87-GL-GFP PDE7B H217Q cells) tumor model. Cancer-induced and transient (with focused ultrasound, FUS) disruption of BBB integrity was monitored with NIR fluorescent dyes. Results: Stereotactic injection of 50,000 cells into mouse brain allowed rapid reestablishment of BBB integrity within a week, as determined by the inability of both tumor-targeted and generic NIR imaging agents to extravasate into the brain. Tumor-induced BBB disruption was observed 7 weeks after tumor implantation. FUS achieved a similar effect at any time point after reestablishing BBB integrity. While tumor uptake and retention of the passive NIR dye, indocyanine green, was negligible, both actively tumor-targeting agents exhibited selective accumulation in the tumor region. The tumor-targeting molecular probe that clears rapidly from nontumor brain tissue exhibits higher contrast than the analogous vascular-targeting agent and helps delineate tumors from sham control. Conclusions: We highlight the utility of FMT imaging for longitudinal assessment of brain tumors and the interplay between the stages of BBB disruption and molecular probe retention in tumors, with potential application to other neurological diseases.

Proceedings Article | 20 February 2018 Presentation + Paper

Presentation + Paper

Ultrasmall visible-to-near-infrared emitting silver-sulfide quantum dots for cancer detection and imaging

Rui Tang, Baogang Xu, Duanwen Shen, Gail Sudlow, Samuel Achilefu

Proceedings Volume 10508, 105080G (2018) https://doi.org/10.1117/12.2300944

KEYWORDS: Tumors, Silver, Luminescence, Near infrared, Nanoparticles, In vivo imaging, Cancer, Toxicity, Quantum dots, Nanomaterial synthesis

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Proceedings Article | 20 February 2018 Paper

Paper

Effect of linkers on the αvβ3 integrin targeting efficiency of cyclic RGD-conjugates.

Partha Karmakar, Dorota Grabowska, Gail Sudlow, Kostiantyn Ziabrev, Nibedita Sanyal, Samuel Achilefu

Proceedings Volume 10508, 1050809 (2018) https://doi.org/10.1117/12.2301223

KEYWORDS: Tumors, In vivo imaging, Luminescence, Confocal microscopy, Cancer, Breast cancer, Chemistry, Near infrared, Imaging systems, Near infrared spectroscopy

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Proceedings Article | 26 April 2016 Presentation

Presentation

A compact bio-inspired visible/NIR imager for image-guided surgery (Conference Presentation)

Shengkui Gao, Missael Garcia, Chris Edmiston, Timothy York, Radoslav Marinov, Suman Mondal, Nan Zhu, Gail Sudlow, Walter Akers, Julie Margenthaler, Rongguang Liang, Marta Pepino, Samuel Achilefu, Viktor Gruev

Proceedings Volume 9696, 96960A (2016) https://doi.org/10.1117/12.2214617

KEYWORDS: Imaging systems, Optical filters, Biomimetics, Near infrared, Image-guided intervention, Visual system, Optical sensors, Tumors, Breast cancer, Surgery

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SPIE Journal Paper | 4 December 2014 Open Access

Open Access

Trimodal color-fluorescence-polarization endoscopy aided by a tumor selective molecular probe accurately detects flat lesions in colitis-associated cancer

Tauseef Charanya, Timothy York, Sharon Bloch, Gail Sudlow, Kexian Liang, Missael Garcia, Walter Akers, Deborah Rubin, Viktor Gruev, Samuel Achilefu

JBO, Vol. 19, Issue 12, 126002, (December 2014) https://doi.org/10.1117/12.10.1117/1.JBO.19.12.126002

KEYWORDS: Polarization, Luminescence, Tissues, Endoscopy, Tumors, Colon, Endoscopes, Near infrared, Tissue optics, In vivo imaging

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Proceedings Article | 4 March 2014 Paper

Paper

Intraoperative imaging and fluorescence image guidance in oncologic surgery using a wearable fluorescence goggle system

Suman Mondal, Shengkui Gao, Nan Zhu, Yang Liu, Gail Sudlow, Walter Akers, Rongguang Liang, Viktor Gruev, Samuel Achilefu

Proceedings Volume 8936, 89360P (2014) https://doi.org/10.1117/12.2039903

KEYWORDS: Imaging systems, Surgery, Goggles, Luminescence, Tumors, Tissues, Near infrared, Cancer, Head-mounted displays, Visualization

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Proceedings Article | 28 February 2014 Paper

Paper

Quantitative determination of maximal imaging depth in all-NIR multiphoton microscopy images of thick tissues

Pinaki Sarder, Walter Akers, Gail Sudlow, Siavash Yazdanfar, Samuel Achilefu

Proceedings Volume 8948, 894827 (2014) https://doi.org/10.1117/12.2036868

KEYWORDS: Tissues, Kidney, Photon counting, Near infrared, Imaging systems, Multiphoton microscopy, Luminescence, Microscopy, Mirrors, Molecules

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SPIE Journal Paper | 27 November 2013 Open Access

Open Access

Dynamic optical projection of acquired luminescence for aiding oncologic surgery

Pinaki Sarder, Kyle Gullicksrud, Suman Mondal, Gail Sudlow, Samuel Achilefu, Walter Akers

JBO, Vol. 18, Issue 12, 120501, (November 2013) https://doi.org/10.1117/12.10.1117/1.JBO.18.12.120501

KEYWORDS: Luminescence, Surgery, Tumors, Projection systems, Visualization, Lymphatic system, Imaging systems, Digital Light Processing, Tissues, Medicine

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SPIE Journal Paper | 22 October 2013 Open Access

Open Access

All-near-infrared multiphoton microscopy interrogates intact tissues at deeper imaging depths than conventional single- and two-photon near-infrared excitation microscopes

Pinaki Sarder, Siavash Yazdanfar, Walter Akers, Rui Tang, Gail Sudlow, Christopher Egbulefu, Samuel Achilefu

JBO, Vol. 18, Issue 10, 106012, (October 2013) https://doi.org/10.1117/12.10.1117/1.JBO.18.10.106012

KEYWORDS: Tissues, Near infrared, Luminescence, Kidney, Multiphoton microscopy, Imaging systems, Confocal microscopy, Microscopy, Microscopes, Control systems

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SPIE Journal Paper | 31 May 2013 Open Access

Open Access

Near-infrared fluorescence goggle system with complementary metal–oxide–semiconductor imaging sensor and see-through display

Yang Liu, Raphael Njuguna, Thomas Matthews, Walter Akers, Gail Sudlow, Suman Mondal, Rui Tang, Viktor Gruev, Samuel Achilefu

JBO, Vol. 18, Issue 10, 101303, (May 2013) https://doi.org/10.1117/12.10.1117/1.JBO.18.10.101303

KEYWORDS: Luminescence, Goggles, Imaging systems, Signal to noise ratio, Near infrared, Signal detection, Liver, Surgery, CMOS technology, Optical sensors

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SPIE Journal Paper | 1 November 2012 Open Access

Open Access

Complementary fluorescence-polarization microscopy using division-of-focal-plane polarization imaging sensor

Yang Liu, Timothy York, Walter Akers, Gail Sudlow, Viktor Gruev, Samuel Achilefu

JBO, Vol. 17, Issue 11, 116001, (November 2012) https://doi.org/10.1117/12.10.1117/1.JBO.17.11.116001

KEYWORDS: Polarization, Tissues, Tumors, Luminescence, Microscopy, Cancer, Optical sensors, Sensors, Microscopes, Near infrared

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Proceedings Article | 28 February 2012 Paper

Paper

Fluorescence goggle for intraoperative breast cancer imaging

Yang Liu, Adam Bauer, Walter Akers, Gail Sudlow, Kexian Liang, Tauseef Charanya, Suman Mondal, Joseph Culver, Samuel Achilefu

Proceedings Volume 8215, 82150G (2012) https://doi.org/10.1117/12.910509

KEYWORDS: Tumors, Luminescence, Goggles, Tissues, Near infrared, Breast cancer, Surgery, Imaging systems, Visualization, Laser safety equipment

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Proceedings Article | 2 February 2012 Paper

Paper

Time-domain imaging with quench-based fluorescent contrast agents

Walter Akers, Metasebya Solomon, Gail Sudlow, Mikhail Berezin, Samuel Achilefu

Proceedings Volume 8233, 82330G (2012) https://doi.org/10.1117/12.915917

KEYWORDS: Luminescence, Tumors, Fluorescence resonance energy transfer, In vivo imaging, Molecules, Quenching (fluorescence), Quantum efficiency, Tissues, Cancer, Near infrared

Read Abstract +

Quench-based probes utilize unique characteristics of fluorescence resonance energy transfer (FRET) to enhance contrast upon de-quenching. This mechanism has been used in a variety of molecular probes for imaging of cancer related enzyme activity such as matrix metalloproteinases, cathepsins and caspases. While non-fluorescent upon administration, fluorescence can be restored by separation of donor and acceptor, resulting in higher intensity in the presence of activator. Along with decreased quantum yield, FRET also results in altered fluorescence lifetime. Time-domain imaging can further enhance contrast and information yield from quench-based probes. We present in vivo time-domain imaging for detecting activation of quench-based probes. Quench-based probes utilize unique characteristics of fluorescence resonance energy transfer (FRET) to enhance contrast upon de-quenching. This mechanism has been used in a variety of molecular probes for imaging of cancer related enzyme activity such as matrix metalloproteinases, cathepsins and caspases. While non-fluorescent upon administration, fluorescence can be restored by separation of donor and acceptor, resulting in higher intensity in the presence of activator. Along with decreased quantum yield, FRET also results in altered fluorescence lifetime. Time-domain imaging can further enhance contrast and information yield from quench-based probes. We present in vivo time-domain imaging for detecting activation of quench-based probes. Time-domain diffuse optical imaging was performed to assess the FRET and quenching in living mice with orthotopic breast cancer. Tumor contrast enhancement was accompanied by increased fluorescence lifetime after administration of quenched probes selective for matrix metalloproteinases while no significant change was observed for non-quenched probes for integrin receptors. These results demonstrate the utility of timedomain imaging for detection of cancer-related enzyme activity in vivo.

SPIE Journal Paper | 1 June 2011 Open Access

Open Access

Detection of enzyme activity in orthotopic murine breast cancer by fluorescence lifetime imaging using a fluorescence resonance energy transfer-based molecular probe

Metasebya Solomon, Kevin Guo, Gail Sudlow, Mikhail Berezin, W. Barry Edwards, Samuel Achilefu, Walter Akers

JBO, Vol. 16, Issue 06, 066019, (June 2011) https://doi.org/10.1117/12.10.1117/1.3594153

KEYWORDS: Luminescence, Tumors, Fluorescence resonance energy transfer, Tissues, Breast cancer, In vivo imaging, Molecular energy transfer, Fluorescence lifetime imaging, Cancer, Near infrared

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Proceedings Article | 12 February 2011 Paper

Paper

Compact intraoperative imaging device for sentinel lymph node mapping

Yang Liu, Adam Bauer, Walter Akers, Gail Sudlow, Kexian Liang, Duanwen Shen, Mikhail Berezin, Joseph Culver, Samuel Achilefu

Proceedings Volume 7910, 79100D (2011) https://doi.org/10.1117/12.886226

KEYWORDS: Lymphatic system, Near infrared, Luminescence, Imaging devices, Surgery, Cancer, Sensors, Light sources, Imaging systems, Tissues

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Showing 5 of 17 publications

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