We report a single plane illumination microscope dedicated to image “Organ-on-chip”-like biostructures in microfluidic systems. By allowing 3D fluorescence imaging inside the chip, it will permit to follow in time sample’s development at cellular scale.
We present herein a microfluidic system based on passive effects for continuously separate a diphasic fluid-particles flow. Initially developed as a portable blood fragmentation device, its ability to operate passively, on several kinds of objects – organic or inorganic – opens the way to environmental applications, such as water cleaning or analysis. This technology can be implemented as a SamplePrep system, first step of an on-site analysis protocol. In addition, its low-cost and reliable manufacturing, compact size, low weight and ease of use make this technology a possible support for the deployment of health policies in developing countries.
The lab-on-a-chip approach has been increasingly present in biological research over the last ten years, high-throughput analyses being one of the promising utilization.
The work presented here has consisted in developing an automated genotyping system based on a continuous flow analysis which integrates all the steps of the genotyping process (PCR, purification and sequencing).
The genotyping device consists of a disposable hybrid silicon-plastic microfluidic chip, equipped with a permanent external, heating/cooling system, syringe-pumps based injection systems and on-line fluorescence detection. High throughput is obtained by performing the reaction in a continuous flow (1 reaction every 6min per channel) and in parallel (48 channels).
We are presenting here the technical solutions developed to fabricate the hybrid silicon-plastic microfluidic device. It includes a polycarbonate substrate having 48 parallel grooves sealed by film lamination techniques to create the channels. Two different solutions for the sealing of the channels are compared in relation to their biocompatibility, fluidic behavior and fabrication process yield. Surface roughness of the surface of the channels is the key point of this step. Silicon fluidic chips are used for thermo-cycled reactions. A specific bonding technique has been developed to bond silicon chips onto the plastic part which ensures alignment and hermetic fluidic connexion. Surface coatings are studied to enhance the PCR biocompatibility and fluidic behavior of the two-phase liquid flow. We then demonstrate continuous operation over more than 20 hours of the component and validate PCR protocol on microliter samples in a continuous flow reaction.
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