We systematically investigate the changes of lipid and protein contents at all developmental stages of Caenorhabditis elegans, including L1 to L4 larvae, and adults, using the recently developed hyperspectral stimulated Raman scattering (hsSRS) microscopy. The gut granules in larvae, which are known as lysosome-like organelles, are specifically identified and the temporal change of these gut granules are tracked at different stages. By acquiring the spectral images of C. elegans at different ages and analyzing the lipid spectra change statistically with hsSRS, we will foster a better understanding of the physiological and pathophysiological processes in living organisms.
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