Several imaging modalities such as optical coherence tomography, photothermal, photoacoustic and magnetic resonance imaging, are sensitive to different physical properties (i.e. scattering, absorption and magnetic) that can provide contrast within biological tissues. Usually exogenous agents are designed with specific properties to provide contrast for these imaging methods. In nano-biotechnology there is a need to combine several of these properties into a single contrast agent. This multifunctional contrast agent can then be used by various imaging techniques simultaneously or can be used to develop new imaging modalities. We reported and characterized a multifunctional nanoparticle, made from gold nanoshells, which exhibits scattering, photothermal, photoacoustic, and magnetic properties.
Gold nanorods (GNRs) have been demonstrated as a scattering imaging agent or therapeutic agent. Because of their
narrow window, biocompatibility, and uniform small size for blood circulation, GNRs are well suited to serve as
imaging contrast agents. Especially, strong phothothermal (PT) effect is attractive for diagnostic (e.g. sentinel lymph
node biopsy) or therapeutic (e.g. PT therapy) purposes. In this paper, we demonstrate GNRs as multipurpose agents with
PT-optical coherence tomography (OCT) for imaging sentinel lymph node. The results show that GNRs are promising
for imaging contrast enhancement for visualizing the detailed functions of SLN.
Sentinel lymph node (SLN) is the first lymph node to drain wastes originated from cancerous tissue. There is a need for
an in vivo imaging method that can image the intact SLN in order to further our understanding of its normal as well as
abnormal functions. We report the use of ultrahigh sensitive optical microangiography (UHS-OMAG) to image
functional microvascular and lymphatic vessel networks that innervate the intact lymph node in mice in vivo. The
promising results show a potential role of UHS-OMAG in the future understanding and diagnosis of the SLN
involvement in cancer development.
KEYWORDS: Ischemia, Optical coherence tomography, Capillaries, Kidney, In vivo imaging, Blood circulation, 3D image processing, Tissue optics, Arteries, Imaging systems
We present a non-invasive, label-free imaging technique called Ultrahigh Sensitive Optical Microangiography (UHSOMAG)
for high sensitive volumetric imaging of renal microcirculation. The UHS-OMAG imaging system is based on
spectral domain optical coherence tomography (SD-OCT), which uses a 47000 A-line scan rate CCD camera to perform
an imaging speed of 150 frames per second that takes only ~7 seconds to acquire a 3D image. The technique, capable of
measuring slow blood flow down to 4 um/s, is sensitive enough to image capillary networks, such as peritubular
capillaries and glomerulus within renal cortex. We show superior performance of UHS-OMAG in providing depthresolved
volumetric images of rich renal microcirculation. We monitored the dynamics of renal microvasculature during
renal ischemia and reperfusion. Obvious reduction of renal microvascular density due to renal ischemia was visualized
and quantitatively analyzed. This technique can be helpful for the assessment of chronic kidney disease (CKD) which
relates to abnormal microvasculature.
Sentinel lymph nodes (SLNs) are the first lymph nodes to drain wastes originated from cancerous tissue. There is a need for an in vivo imaging method that can image the intact SLN to further our understanding of its normal as well as abnormal functions. We report the use of ultrahigh sensitive optical microangiography (UHS-OMAG) to image functional microvascular and lymphatic vessel networks that innervate the intact lymph node in mice in vivo. The promising results show a potential role of UHS-OMAG in the future understanding and diagnosis of the SLN involvement in cancer development.
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