Dr. Xiyu Yi Profile

Dr. Xiyu Yi

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Proceedings Article | 22 February 2019 Presentation + Paper

3D super-resolution imaging using a generalized and scalable progressive refinement method on sparse recovery (PRIS)

Xiyu Yi, Rafael Piestun, Shimon Weiss

Proceedings Volume 10884, 1088406 (2019) https://doi.org/10.1117/12.2506827

KEYWORDS: Point spread functions, Spindles, Microscopy, Molecules, Super resolution, Compressed sensing, 3D image processing, Imaging systems

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Within the family of super-resolution (SR) fluorescence microscopy, single-molecule localization microscopies (PALM[1], STORM[2] and their derivatives) afford among the highest spatial resolution (approximately 5 to 10 nm), but often with moderate temporal resolution. The high spatial resolution relies on the adequate accumulation of precise localizations, which requires a relatively low density of bright fluorophores. Several methods have demonstrated localization at higher densities in both two dimensions (2D)[3, 4] and three dimensions (3D)[5-7]. Additionally, with further advancements, such as functional super-resolution[8, 9] and point spread function (PSF) engineering with[8-11] or without[12] multi-channel observations, extra information (spectra, dipole orientation) can be encoded and recovered at the single molecule level. However, such advancements are not fully extended for high-density conditions in 3D. In this work, we adopt sparse recovery using simple matrix/vector operations, and propose a systematic progressive refinement method (dubbed as PRIS) for 3D high-density condition. We also generalized the method for PSF engineering, multichannel and multi-species observations using different forms of matrix concatenations. Specifically, we demonstrate reconstructions with both double-helix and astigmatic PSFs, for both single and biplane settings. We also demonstrate the recovery capability for a mixture of two different color species.

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