An automated cell detection and sorting system was developed, combining both the optofluidic intracavity spectroscopy
(OFIS) technique and dielectrophoresis (DEP). The OFIS method utilizes a microfluidic channel as a Fabry-Perot cavity
to produce characteristic transmission spectra of individual cells. The concept behind optical detection is that a decrease
in spectral intensity beyond a threshold indicates that a cell is present. Upon detection, an RF voltage is automatically
applied to electrodes, trapping the cell with DEP forces. The system then sorts the cell into one of two microfluidic
channels based on resulting optical analysis. A further advantage is that RF joule heating can be measured from known
dn/dT values of the medium, which is useful for investigating cell viability issues.
The label-free technique of optofluidic intracavity spectroscopy (OFIS) utilizes the optical transmission spectrum of a
cell in a microfluidic Fabry-Pérot (F-P) cavity to distinguish cells from cancerous cell lines and baseline normal blood
cells. The classification between canine hemangiosarcoma (HSA) cancer cells and monocytes in canine normal
peripheral blood mononuclear cells (PBMCs) had been demonstrated with 95% sensitivity and 98% specificity. Now
with a new optical model that treats the cell settled at the bottom of the cavity as a thin lens, the focal length of cells was
extracted and used as an individual cell malignancy indicator.
The label-free technique of optofluidic intracavity spectroscopy (OFIS) uses the optical transmission spectrum of a cell
in a microfluidic optical resonator to distinguish cancerous and non-cancerous cells. Based on their distinctive
characteristic transmission spectra, canine hemangiosarcoma (HSA) cancer cells and normal peripheral blood
mononuclear cells (PBMCs) have been differentiated using the OFIS technique with high statistical significance (p<10-
6). 95% sensitivity and 98% specificity were achieved simultaneously. A cell lens model explains trends in the transverse
mode pattern in the transmission spectra of HSA cells and allows extraction of cell focal length.
A multi-channel fiber optic photoluminescence system was developed for real time, in situ and continuous
quantitative monitoring of chemical parameters in analytes, with the advantages of ease of use and low cost in
manufacturing. The system sequentially excites 8 optodes and measures the resulting photoluminescence with a single
photomultiplier. The oxygen sensitive phosphorescent dye or pH sensitive fluorescent dye in the optodes transduces the
activity of an enzyme genetically engineered to detect a specific chemical species such as a chlorinated ethene. System
operation is described and the relative signal is compared to measurements made with single channel system based on a
commercial spectrometer.
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