With the development of the research on life sciences and clinic medicine diagnose, the fluorescence microscope is requested to not only satisfy subjective observation, but also have computer external analysis. It also is requested to not only offer image conformation information, but also offer image spectrum information. The research of the system is brought forward for satisfying the questions. It found the solutions to that the traditional fluorescence microscope could not offer contiguous adjustable excitation spectrum, and that we could not get digitized fluorescence microscopic image information and spectrum information and so on. The paper detailedly specifies the pivotal technologies of the research of the continuous spectrum analytic system of micro-fluorescence image: the part of excitation lamp-house, the part of micro- fluorescence image acquisition and the part of computer control analysis.
Recently, many efforts have been made to improve the device performance of nanocrystal memory by replacing the SiO2 with various high dielectric constant (high-k) materials, especially embedded with Ge nanocrystals. This paper demonstrates the floating gate memory effect by embedding nanometer-sized Ge nanocrystals in hafnium aluminate (HfAlO) high-k gate dielectric. A 5 nm-thick amorphous thin film of HfAlO was first deposited on (100) p-Si substrates as a tunneling gate oxide layer by laser molecular beam epitaxy deposition using a HfO2 and Al2O3 composite target. Well-defined (~10 nm in diameter) nanometer-sized Ge dots were subsequently deposited on this thin tunneling gate oxide followed by a 30 nm-thick top control gate oxide of HfAlO. Transmission electron microscopy has been carried out for a detailed study of structural properties of the Ge nanocrystals embedded in the HfAlO films, and their relationships to electrical properties. Electrical properties have been characterized by means of high-frequency capacitance-voltage (C-V) and current-voltage (I-V) measurements on the metal-oxide-semiconductor capacitors. A counter-clockwise hysteresis C-V loop has been obtained and a threshold voltage shift of 1.0 V has been achieved indicating stored electrons (up to a density of 2x1012 cm-2) in the Ge-nanodots floating gate and thus the memory effect. Time-dependent I-V measurement also showed low leakage current of floating gate system. These results suggest that the Ge nanocrystals embedded in HfAlO are promising for floating gate memory device application.
This paper is centered on the development of reagent-free method based on Fourier transform mid infrared (FT-MIR), near infrared red (NIR) and Fourier transform (FT)-Raman spectroscopy. Suitable spectral wavenumber regions were selected for principal least square (PLS) regression and calibration models developed. In MIR, urine sample measured with Horizontal ATR, which is the accessory of Perkin-Elmer Spectrum GX Spectrometer, and the important range is 900-1800 cm-1. NIR spectra are obtained for each sample with 1mm pathlength. Absorption spectra of urine samples in 5000~4200cm-1 is used for PLS regression. The Raman spectra were recorded with a Bruker IFS 66 spectrometer with an FRA 106 FT-Raman accessory unit. The FT-Raman spectra of albumin power and albumin solution is recorded. The main noise is form water. The root mean square error of prediction (RMSEP) of glucose is 34.9 mg/dL(MIR), 31.56mg/dL(NIR) , and the RMSEP of albumin is 23.2mg/dL (MIR), 17.9mg/dL(NIR). Result indicated that FT-MIR and NIR spectroscopy could be used for rapid analysis of urine. FT-Raman spectroscopy could applied for compositional identification.
The technology of computer analysis for gel electrophoresis images is based on the computer image analysis and gel electrophoresis. Particle in complex mixture have different molecular weight and with different charge. Migration distances of sample zones are compared with the position of standards and the molecular size of unknowns is determined. By this way, biology particle and molecule with different compound can be separated from other. Digital camera can acquire image and process digital transact, then based on the digital image to realize automatic identification, sign, molecular weight calculate. For the convenient of observe and acquire, sample pre band on ethidium bromide stained agarose gels. The instrument is mainly made up of UV light source, digital camera and camera obscura etc. Formulas for calculate of protein's molecular weight and mobility in polyacrylamide gel is connected with standard curve and the relative mobility of band. After many experiments the conclusion can be deduced that the change of electrophoresis start point and front edge (m, n, r's value) will result in the change of K and b. Unknown samples' molecular weight only have relation with the standard sample's molecular weight and the value of r1, r2 and r3.
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