Dr. Stéphanie Hambÿe Profile

Dr. Stéphanie Hambÿe

at Univ de Mons

SPIE Involvement:

Author

Publications (1)

This will count as one of your downloads.

You will have access to both the presentation and article (if available).

DOWNLOAD NOW

This content is available for download via your institution's subscription. To access this item, please sign in to your personal account.

Email or Username Forgot your username?

Password Forgot your password?

Show

Keep me signed in

No SPIE account? Create an account

Proceedings Article | 17 May 2022 Presentation + Paper

PfHRP2 detection using plasmonic optical fibers: a step towards early malaria diagnosis

Médéric Loyez, Mathilde Wells, Stéphanie Hambÿe, François Hubinon, Bertrand Blankert, Ruddy Wattiez, Christophe Caucheteur

Proceedings Volume 12139, 121390Q (2022) https://doi.org/10.1117/12.2620951

KEYWORDS: Optical fibers, Proteins, Blood, Plasmonics, Gold, Biosensors, Sensors, Target detection, Spectroscopy, Multiplexing

Read Abstract +

Biosensing using optical fibers allows the detection of low concentrations of analytes. To exploit this property, we study a cost-effective fiber-based solution to target the presence of Plasmodium falciparum histidine-rich protein 2 (PfHRP2), a biomarker for malaria diagnosis. In this work, unclad multimode optical fiber probes (400 µm core diameter) are coated with a thin gold film to excite Surface Plasmon Resonance (SPR), yielding high sensitivity to surrounding medium and more precisely to target-bioreceptor interactions. They are connected to a portable white light source and a spectrometer for read-out. The covalent immobilization of anti-PfHRP2 antibodies on gold and its passivation allowed specific detection in different types of media. The use of secondary antibodies as amplifiers in a sandwich assay also allowed us to improve the sensitivity of the method. Different blocking agents (fish gelatin, bovine serum albumin and casein) were studied to optimize the surface selectivity. The detection of PfHRP2 was first calibrated using spiked proteins into phosphate buffer saline (PBS) and then tested in vitro using fresh cultures of Plasmodium falciparum. The shifts observed with our technique were also compared with results obtained from an ELISA (gold-standard technique for detection and quantification of targets), and commercial paper-based lateral-flow assays, usually used on field. As malaria affects many people worldwide, improvement and multiplexing of this technique can be a perspective to overcome the limitations of currently available point-of-care tests in the future.

My Library

You currently do not have any folders to save your paper to! Create a new folder below.

Folder Name

Folder Description

View contact details

UPDATE YOUR PROFILE

Is this your profile? Update it now.

Sign into your SPIE.org account

Don’t have a profile and want one?

Create an account on SPIE.org

Access to the requested content is limited to institutions that have purchased or subscribe to SPIE eBooks. You are receiving this notice because your organization may not have SPIE eBooks access.*

*Shibboleth/Open Athens users─please sign in to access your institution's subscriptions.

To obtain this item, you may purchase the complete book in print or electronic format on SPIE.org.

ORGANIZATIONAL
Sign in with credentials provided by your organization.

Organizational Username

Organizational Password

Show/Hide Password

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

;

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members:

Non-members: ADD TO CART

Keywords/Phrases

Search In:

Publication Years