Laser interferometry for measuring micromovements of the eyeball has been investigated. A technique for processing an interference signal is proposed, which makes it possible to obtain graphs of the speed and acceleration of the eyeball through the closed eyelid. The unknown motion parameters are found from the frequency of the interference signal of the self-mixing laser system as a result of the windowed Fourier transform. The RLD78NZM5 module was used as a source of laser radiation. Experimental measurements were carried out to determine the parameters of eye movement on volunteers at the age of 20 years. For the first volunteer in a calm state, the velocities of the eyeball movement were in the range of up to 800 μm/s, and the accelerations were in the range of ±20 μm/s2. For the second volunteer in a calm state, the velocities of the movement of the eyeball were in the range of up to 1300 μm/s, and the accelerations were in the range of up to 40 μm/s2. The speed and acceleration of eyeball movements of the volunteers at rest had the lowest values. These parameters increased with eye movement. When the eye moved to the left and to the right, the speed of the eyeball movement of the first volunteer was higher than when the eye moved up and down. On the contrary, when the eye moved to the left and to the right, the velocities of the eyeball movement of the second volunteer were less than when the eye moved up and down. In addition, the analysis of the movement of the eyeball can be carried out even in its closed state. It has been shown that the physiological state of the somebody can influence the nature of eye movement. This influence can be used to assess the psychoemotional state and diagnose various pathologies of the oculomotor apparatus of the human body.
Our previous study has revealed that specific ornithosis antigens extracted from Сhlamydia psittaci (CP) could possess some modulatory activity on the growth of malignant solid tumors in Wistar rats. Here, in the same model we studied dose-dependent dynamics of tumor growth under the influence of CP antigen derived from CP AMK-16 strain, in doses ranged from 0.025 mg/kg to 1.25 mg/kg given as a single subcutaneous injection. Velocity of tumor growth has been estimated by t-LASCA technique.
In this report, the influence of C. psittaci (CP) antigens on the process of a development of a malignant solid tumor in the inbred Wistar white rats in experimental conditions has been successfully studied. Velocity of growing of a tumor has been estimated by s-LASCA technique. Measurement of statement of a tumor has been performed daily after each of three serial injections of the CP specific ornithosis antigen derived from two different CP strains, the Rostinovo-70 and the АМК. Single injection solutions were administered in a volume of 1 ml. We found, that both antigens could initiate the marked decrease of the growth of malignant tumors in the animal model used.
A method for measuring acceleration based on the use of the fast-discrete Fourier transform algorithm is proposed. The simulation of the spectrum of the self-mixing signal at the uniformly accelerated motion of the reflector is carried out. The relationship of low-frequency and high-frequency components of the self-mixing signal spectrum with the object acceleration value is shown. The measurement of uniformly accelerated motion of an object on the spectrum of a self-mixing signal is experimentally realized. The accelerated motion of the reflector was carried out using a signal generator built into the laboratory station of the Ni ELVIS virtual instruments. The results of measuring the motion of piezoceramics with the acceleration given by the quadratic law of voltage change on it are presented. The results of calculation of acceleration on the spectrum of the self-mixing signal for 26 μm/s2 are presented. The resolution of the proposed method was estimated by measuring the frequencies of neighboring spectral components and amounted to 500 nm/s2.
Formation of output signal of device, realizing Doppler technique, has been studied. Naïve embryo (non-infected fertilized chicken eggs) has been used as a test object. Dependence of first frequency-weighted spectral moment of output signal on the day of observation of chicken embryo has been investigated. The possibility of adaption of the Doppler diagnostics for monitoring of viability and detection of pathology of development of chicken embryo, infected with Chlamydia trachomatis cells, is discussed.
Laser self-mixing interferometry of absolute distances is represented by the method of frequency modulation of supply current of the laser diode (FMCW technique). The advantages of the triangular wave modulation method in combination with the use of the frequency of the self-mixing signal spectrum have been shown. The advantages of the harmonic wave modulation method in combination with the use of the some various amplitudes of the self-mixing signal spectrum have been shown were shown also. The advantages of the harmonic wave modulation method in combination with the use of different amplitudes of the spectrum of self-mixing signals are shown. The equipment includes a frequency-modulated semiconductor self-mixing laser diode RLD-650 on quantum-size structures with a diffraction-limited single spatial mode with a wavelength of 654 nm. The results of computer simulation showed that the accuracy of determining the distance at the harmonic wave modulation decreases with increasing the distance to the measured object. But at small distances, its value is much smaller than at the triangular wave modulation of laser radiation.
Pulse wave from naïve fertilized chicken eggs has been clearly detected using laser photoplethysmography. Highfrequency modulation of intensity of illuminating laser light has been used to enhance output signal of experimental setup and to increase the S/N ratio. The form of pulse wave has been analyzed for naïve embryo on different stages of their development.
The possibilities of self-mixing interferometry for measuring nanodisplacement of a probe included in a near-field scanning microwave microscope have been considered. The features of the formation of a laser interference signal at current modulation of the wavelength of laser radiation have been investigated. Experimental responses of a semiconductor laser system included in scanning microwave microscope to control nanodisplacement of the probe have been demonstrated.To register the nanodisplacement of the probe, it is proposed to use the method of determining the stationary phase of a laser interference signal by low-frequency spectrum of a semiconductor laser. The change of the amplitudes of the spectral components in the spectrum of the interference signal due to creation of the standing wave in the external resonator of the laser self-mixing system has been shown. The form of the interference signal at current modulation of the radiation wavelength was experimentally obtained when the probe moves with a step of 80 nm. The results of measuring nanodisplacements of an electromagnetic translator STANDA 8MVT40-13 have been demonstrated. Deviation of the nanodisplacement of the proposed method does not exceed 15%.
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