Cystic fibrosis (CF) is a condition arising from a genetic disorder of the cystic fibrosis transmembrane conductance regulator (CFTR) protein traversing luminal organ epithelial lining. Nasal potential difference (NPD) measurement can be used to study CF through evaluation of CFTR channel activity. Salt-bridge-based probes in combination with calomel electrodes have been used to provide discriminative information between normal and CF patients. This setup is bulky and less convenient for use in a clinical setting. Thus, we have developed a small caliber NPD probe and validated it in 3 normal subjects, providing results consisted to those reported in literature.
Our lab has developed a 2-mm-diameter transnasal introduction tube (TNIT) that enables safe and rapid optical coherence tomography (OCT) imaging of the upper gastrointestinal tract in unsedated pregnant women. Here, we report our clinical experience with TNIT-based OCT imaging in unsedated pregnant women (n=5) at Mass General Hospital (MGH). Results show that OCT imaging of the esophagus, stomach, and duodenum can be safely and effectively conducted in pregnant women with this device.
We have previously demonstrated a miniaturized transnasal introduction tube (TNIT) for transnasal endomicroscopy (TNEM) with optical coherence tomography (OCT) for clinical imaging of the small intestine of infants and adults in vivo. Although the TNIT is a convenient and effective way to implement TNEM, OCT probes for imaging through the TNIT had long manufacturing times and low yields, and its multiple cylindrical surfaces caused severe optical aberrations, degrading OCT image quality. Here we introduce a new optical design for 3D-printed microoptics that correct TNIT-induced astigmatism. Preliminary results show that the lens improves resolution and can be reliably manufactured.
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