Our previous study has revealed that specific ornithosis antigens extracted from Сhlamydia psittaci (CP) could possess some modulatory activity on the growth of malignant solid tumors in Wistar rats. Here, in the same model we studied dose-dependent dynamics of tumor growth under the influence of CP antigen derived from CP AMK-16 strain, in doses ranged from 0.025 mg/kg to 1.25 mg/kg given as a single subcutaneous injection. Velocity of tumor growth has been estimated by t-LASCA technique.
In this report, the influence of C. psittaci (CP) antigens on the process of a development of a malignant solid tumor in the inbred Wistar white rats in experimental conditions has been successfully studied. Velocity of growing of a tumor has been estimated by s-LASCA technique. Measurement of statement of a tumor has been performed daily after each of three serial injections of the CP specific ornithosis antigen derived from two different CP strains, the Rostinovo-70 and the АМК. Single injection solutions were administered in a volume of 1 ml. We found, that both antigens could initiate the marked decrease of the growth of malignant tumors in the animal model used.
Formation of output signal of device, realizing Doppler technique, has been studied. Naïve embryo (non-infected fertilized chicken eggs) has been used as a test object. Dependence of first frequency-weighted spectral moment of output signal on the day of observation of chicken embryo has been investigated. The possibility of adaption of the Doppler diagnostics for monitoring of viability and detection of pathology of development of chicken embryo, infected with Chlamydia trachomatis cells, is discussed.
Method of speckle-microscopy has been adapted to the problem of detection of Chlamydia trachomatis microbial cells in aqueous suspension and fixed on the glass. Combined system “C. trachomatis bacterial cell”-“monoclonal antibody”- “gold nanoparticle” has been used as a complicated scattering element in the case of formation of biospeckles with a small number of scatterers. Optical model of diffraction of strongly focused Gaussian beam on combined scattering system, containing gold nanoparticles, has been suggested. Simulation of speckles, forming with a small number of scattering nanoparticles, has been carried out. It has been demonstrated that the usage of nanoparticles allows to enhance essentially the output signal of speckle-microscope.
Pulse wave from naïve fertilized chicken eggs has been clearly detected using laser photoplethysmography. Highfrequency modulation of intensity of illuminating laser light has been used to enhance output signal of experimental setup and to increase the S/N ratio. The form of pulse wave has been analyzed for naïve embryo on different stages of their development.
Prototype of laser scanning speckle-microscope has been designed. It has been demonstrated experimentally, that signal of speckle microscope is increased drastically in the presence of gold nanoparticles. It has been demonstrated the unique possibility of detection of a single C. trachomatis cell in a tested sample using speckle-microscopy.
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