The study interactions between tumor cells and lymph nodes (LNs), will be investigated with the development of complex 3D cell structures in a microfluidic chip by Laser Induced Forward Transfer (LIFT) technique. The lymphatic system and LNs are an integral part of our adaptive immune system and many tumors exploit lymphatic vessels to spread and colonize downstream LNs. The complex cell structures will be realized by the printing of tumor organoids and the multilayer printing of 2D layers of LN cells, initially on ECM and other substrates, and ultimately inside a microfluidic chip.
Bioprinting is a rapidly expanding additive manufacturing process, for the development of complex biostructures such as tissue-like structures, able to imitate native tissue functions. Among the most commonly used bioprinting techniques, Laser Induced Forward Transfer (LIFT) offers the highest degree of spatial resolution (minimum feature of <10 μm) and post-printing cell viability while it has also been used for the immobilization of biomaterials on a variety of substrates. Furthermore, this study will present the control of the depth of cell deposition within the ECM by tuning the laser printing parameters and the organoid formation of the laser printed cells, already from Day 1 after bioprinting. Finally, the application of LIFT technique in Organ on Chip applications will be presented.
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