Dr. Konstantin Grygoryev Profile

Dr. Konstantin Grygoryev

at Tyndall National Institute

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Author

Publications (7)

SPIE Journal Paper | 30 November 2023 Open Access

Laser absorption spectroscopy measurements of different pulmonary oxygen gas concentrations in transmittance and remittance geometry: phantom study

Andrea Pacheco, Jean Matias, Konstantin Grygoryev, Martin Hansson, Sara Bergsten, Stefan Andersson-Engels

JBO, Vol. 28, Issue 11, 115003, (November 2023) https://doi.org/10.1117/12.10.1117/1.JBO.28.11.115003

KEYWORDS: Lung, Sensors, Oxygen, Absorption spectroscopy, Tissues, Optical properties, Absorption, Transmittance, Laser spectroscopy, Signal to noise ratio

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SPIE Journal Paper | 5 September 2023 Open Access

Frameworks of wavelength selection in diffuse reflectance spectroscopy for tissue differentiation in orthopedic surgery

Celina Li, Carl Fisher, Katarzyna Komolibus, Konstantin Grygoryev, Huihui Lu, Ray Burke, Andrea Visentin, Stefan Andersson-Engels

JBO, Vol. 28, Issue 12, 121207, (September 2023) https://doi.org/10.1117/12.10.1117/1.JBO.28.12.121207

KEYWORDS: Bone, Tissues, Principal component analysis, Cements, Diffuse reflectance spectroscopy, Surgery, Data modeling, Education and training, Feature selection, Absorption

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Proceedings Article | 6 March 2023 Presentation

GASMAS for the non-invasive monitoring of oxygen gas in healthy neonates

Sri Rama Pranav Kumar Lanka, Jurate Panaviene, Konstantin Grygoryev, Sanathana Konugolu Venkata Sekar, Eugene Dempsey, Stefan Anderresson-Engels

Proceedings Volume PC12368, PC123680N (2023) https://doi.org/10.1117/12.2651130

KEYWORDS: Oxygen, Lung, Scattering media, Chest, Absorption spectroscopy

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SPIE Journal Paper | 5 January 2022 Open Access

Perspective on the integration of optical sensing into orthopedic surgical devices

Carl Fisher, James Harty, Albert J. Yee, Celina Li, Katarzyna Komolibus, Konstantin Grygoryev, Huihui Lu, Ray Burke, Brian Wilson, Stefan Andersson-Engels

JBO, Vol. 27, Issue 01, 010601, (January 2022) https://doi.org/10.1117/12.10.1117/1.JBO.27.1.010601

KEYWORDS: Surgery, Bone, Optical sensing, Tissue optics, Integrated optics, Tissues, Optical properties, Robotics, Soft tissue optics, Scattering

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SPIE Journal Paper | 1 November 2021 Open Access

Lung tissue phantom mimicking pulmonary optical properties, relative humidity, and temperature: a tool to analyze the changes in oxygen gas absorption for different inflated volumes

Andrea Pacheco, Konstantin Grygoryev, Walter Messina, Stefan Andersson-Engels

JBO, Vol. 27, Issue 07, 074707, (November 2021) https://doi.org/10.1117/12.10.1117/1.JBO.27.7.074707

KEYWORDS: Capillaries, Lung, Absorption, Optical properties, Tissue optics, Liquids, Humidity, Sensors, Data acquisition, Tissues

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Significance: Gas in scattering media absorption spectroscopy (GASMAS) enables noninvasive gas sensing in the body. It is developing as a tool for diagnosis and monitoring of respiratory conditions in neonates. Phantom models with relevant features to the clinical translation of GASMAS technology are necessary to understand technical challenges and potential applications of this technique. State-of-the-art phantoms designed for this purpose have focused on the optical properties and anthropomorphic geometry of the thorax, contributing to the source–detector placement, design, and optimization. Lung phantom mimicking the alveolar anatomy has not been included in the existent models due to the inherent complexity of the tissue. We present a simplified model that recreates inflated alveoli embedded in lung phantom. Aim: The goal of this study was to build a lung model with air-filled structures mimicking inflated alveoli surrounded by optical phantom with accurate optical properties (μa = 0.50 cm − 1 and μs′=5.4 cm−1) and physiological parameters [37°C and 100% relative humidity (RH)], and to control the air volume within the phantom to demonstrate the feasibility of GASMAS in sensing changes in pulmonary air volume. Approach: The lung model was built using a capillary structure with analogous size to alveolar units. Part of the capillaries were filled with liquid lung optical phantom to recreate scattering and absorption, whereas empty capillaries mimicked air filled alveoli. The capillary array was placed inside a custom-made chamber that maintained pulmonary temperature and RH. The geometry of the chamber permitted the placement of the laser head and detector of a GASMAS bench top system (MicroLab Dual O2 / H2O), to test the changes in volume of the lung model in transmittance geometry. Results: The lung tissue model with air volume range from 6.89 × 10 − 7 m3 to 1.80 × 10 − 3 m3 was built. Two measurement sets, with 10 different capillary configurations each, were arranged to increase or decrease progressively (in steps of 3.93 × 10 − 8 m3) the air volume in the lung model. The respective GASMAS data acquisition was performed for both data sets. The maximum absorption signal was obtained for configurations with the highest number of air-filled capillaries and decreased progressively when the air spaces were replaced by capillaries filled with liquid optical phantom. Further studies are necessary to define the minimum and maximum volume of air that can be measured with GASMAS-based devices for different source–detector geometries. Conclusions: The optical properties and the structure of tissue from the respiratory zone have been modeled using a simplified capillary array immersed in a controlled environment chamber at pulmonary temperature and RH. The feasibility of measuring volume changes with GASMAS technique has been proven, stating a new possible application of GASMAS technology in respiratory treatment and diagnostics.

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Proceedings Article | 1 March 2019 Paper

Fluorescence spectroscopy of mouse organs using ultraviolet excitation: towards assessment of organ viability for transplantation

Marcelo Saito Nogueira, Katarzyna Komolibus, Konstantin Grygoryev, Jacqueline Gunther, Stefan Andersson-Engels

Proceedings Volume 10876, 1087606 (2019) https://doi.org/10.1117/12.2510966

KEYWORDS: Biomedical optics, Clinical research, Optical diagnostics, Luminescence, Tissues, Liver, Fluorescence spectroscopy, Ultraviolet radiation, Kidney, Lung, Transplantation, Tissue optics, Absorption

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Identifying diseases and evaluating tissue function and viability can be performed by subjective or objective methods. However, subjective techniques may be inaccurate and non-optical objective techniques may be relatively expensive and time-consuming. Then, these techniques may not be suitable for clinical applications that require immediate assessment and intervention. Fluorescence spectroscopy (FS) is one of the optical techniques with great potential for medical diagnostics and surgical guidance. This potential is associated to the possibility of label-free techniques biochemical sensitivity without contrast agents. For clinical applications, fluorescence can be used to assess biomolecular content of respiratory metabolism involving NAD(P)H and FAD. In addition, changes in collagen, elastin, porphyrin, pyridoxine, and tryptophan content can potentially be detected. One way to collect epifluorescence signals from superficial tissue layers is using ultraviolet (UV) excitation. In this study, we used UV excitation FS to investigate the effect of temperature variation (from 0 to 25 degrees Celsius) on tissue autofluorescence. The measurements reproducibility was assessed by variations of the spectral shape accounted by the calculation of the Pearson correlation coefficient for each pair of measurements. Overall, fluorescence measurements were more reproducible at 25°C compared to 0°C. Liver showed lowest fluorescence variability (most homogeneous organ) regarding results from both 300 nm and 340 nm excitations. We report temperature and wavelength-dependent spectral changes due to the tissue thawing by calculating the difference between normalized UVEFS measurements at 0°C and 25°C. Observed differences may be attributed to blood absorption and NADH fluorescence emission. Our results can be used to increase the database of tissue fluorescence spectra using UV excitation for future reference to choose targeted wavelengths in fluorescence instrumentation. Furthermore, our study illustrates expected fluorescence variations during the assessment of organs viability for transplantation, especially due to cold preservation.

Proceedings Article | 17 May 2018 Paper

Diffuse reflectance spectroscopy for determination of optical properties and chromophore concentrations of mice internal organs in the range of 350 nm to 1860 nm

Marcelo Saito Nogueira, Michael Raju, Jacqueline Gunther, Konstantin Grygoryev, Katarzyna Komolibus, Huihui Lu, Stefan Andersson-Engels

Proceedings Volume 10685, 106853G (2018) https://doi.org/10.1117/12.2306636

KEYWORDS: Tissues, Scattering, Reflectivity, Heart, Liver, Mie scattering, Kidney, Chromophores, Optical properties, Rayleigh scattering, Diffuse reflectance spectroscopy, Optical diagnostics, Biomedical optics, Biophotonic applications, Monte Carlo methods

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