Coherent excitonic coupling in a dimer of a GFP-analogue, Venus-A206, was discovered previously by using circular dichroism and a combination of photon antibunching and FCS. To further elucidate this effect, here we use a new approach based on the measurements of the two-photon polarization ratio (Omega) as a function of excitation wavelength. In a Venus tandem dimer we observe significant changes of Omega across the spectrum, compared to a negative control with only one chromophore. Our model suggests that only a fraction of dimers are coupled excitonically. Their constituent dipoles make an angle of ~40 degree in an oblique arrangement.
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