We present a multiscale imaging system in which a conventional microscope stand is inserted in the sample arm of a Spectral-Domain Optical Coherence Microscopy (SD-OCM) system. The instrument was designed to facilitate localisation and maturity assessment of oocytes and early-stage embryos to be used for in vitro fertilisation (IVF), by a versatile and easy switch of microscope objectives. The dispersion variation due to change of the microscope objectives is dealt with by employing the Complex Master Slave procedure that enables correct operation without the use of matching glass in the reference arm for dispersion compensation.
The most successful methods for speckle reduction in Optical Coherence Tomography (OCT) are based on deformation of the wavefront used in scanning. Here, a simple method is presented where the wavefront is distorted by lateral translation of the lens between the 2D galvo-scanner and sample. The report demonstrates that the method can be implemented with a small piezoelectric transducer. Up to 33% improvement in Speckle Contrast Ratio (SCR) of B-scan OCT images is demonstrated.
We present a optical coherence tomography system for minimally-disturbance imaging of porcine embryos, placed inside an incubator which ensures adequate environmental parameters including temperature, humidity, and gas ratios.
A detailed assessment of embryo development would assist biologists with selecting the most suitable embryos for transfer leading to higher pregnancy rates. Currently, only low resolution microscopy is employed to perform this assessment. Although this method delivers some information on the embryo surface morphology, no specific details are shown related to its inner structure. Using a Master-Slave Swept-Source Optical Coherence Tomography (SS-OCT), images of bovine embryos from day 7 after fertilization were collected from different depths. The dynamic changes inside the embryos were examined, in detail and in real-time from several depths. To prove our ability to characterize the morphology, a single embryo was imaged over 26 hours. The embryo was deprived of its life support environment, leading to its death. Over this period, clear morphological changes were observed.
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