KEYWORDS: Sum frequency generation, Collagen, Vibration, Proteins, Mid-IR, Crystals, Chemical elements, Visualization, Signal generators, Raman spectroscopy
Vibrational sum-frequency generation (SFG) microscopy is a highly sensitive and selective optical imaging technique capable of retrieving information on the molecular structure of proteins. In this study, we determined the achiral tensor elements Х_ijk^((2)) associated with the the symmetric and asymmetric methylene (CH2) stretching modes of collagen type I through polarization-sensitive SFG measurements, and compared the results with computational modeling based on the crystal structure information from the Protein Data Bank. These findings contribute to our understanding of protein structure and pave the way to predict proteins' non-linear optical properties directly from their crystal structure.
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