A surface-enhanced Raman spectroscopy (SERS) substrate for saliva analysis was explored to create a non-invasive tool for pediatric eosinophilic esophagitis monitoring during treatment. Herein, the SERS substrate was developed via the in situ growth of nanoparticles within a paper-fluidic platform. The SERS signal enhancement from different metallic surfaces grown within a filter paper was explored to optimize the signal response.
Coupling of surface-enhanced Raman spectroscopy (SERS) with the coffee ring effect can overcome the poor reproducibility typically seen when using SERS. In this study, we developed a nitrocellulose membrane paper-based substrate for coffee ring enhanced SERS, which was highly hydrophobic and produced consistent coffee rings. After optimization of solution parameters including gold nanoparticle concentration and solvent, this platform demonstrated high enhancement and low variability using Malachite Green Isothiocyanate and Moraxella catarrhalis. This substrate has the potential to increase the usability and implementation of SERS by overcoming intrinsic limitations and is more accessible than current substrates.
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