Microwave photonic channelized receiver is one of the important means to realize broadband radio frequency (RF) signal reception, and it also has great application potential in the fields of radar systems and electronic warfare. In view of the above, a microwave photonic channelizer with 66-subchannels based on acousto-optic frequency shifter (AOFS) is proposed. The 11-line optical frequency comb (OFC) used in this scheme is generated via one phase modulator. The 33 optical local oscillators can be obtained using two AOFSs to shift the frequency of 11-line OFC up and down. The 33 optical local oscillators and broadband RF signal are image-rejected and downconverted in the I  /  Q receiver and output by 66 subchannels with a bandwidth of 500 MHz. The simulation results verify the complete reception of 3 to 36 GHz wideband RF signal, and the spurious-free dynamic range of the system can reach 116.3  dB  ·  Hz^2/3.