Super-resolution quantitative phase-contrast imaging by microsphere-based digital holographic microscopy

Qiaowen Lin; Dayong Wang; Yunxin Wang; Sha Guo; Spozmai Panezai; Liting Ouyang; Lu Rong; Jie Zhao

doi:10.1117/1.OE.56.3.034116

25 March 2017 Super-resolution quantitative phase-contrast imaging by microsphere-based digital holographic microscopy

Qiaowen Lin, Dayong Wang, Yunxin Wang, Sha Guo, Spozmai Panezai, Liting Ouyang, Lu Rong, Jie Zhao

Author Affiliations +

Funded by: National Natural Science Foundation of China (NSFC), National Natural Science Foundation of China, Natural Science Foundation of China, Science and Technology Project of Beijing Municipal Commission of Education, Beijing Municipal Institutions, Importation Development of High-Caliber Talents Project of Beijing Municipal Institutions, Centre and Beijing Municipal Natural Science Foundation

Optical Engineering, Vol. 56, Issue 3, 034116 (March 2017). https://doi.org/10.1117/1.OE.56.3.034116

Abstract

A super-resolution quantitative phase-contrast imaging method using high refractive index microsphere is developed to overcome the diffraction limit of optical field, which is produced by the object in the digital holographic microscopy. A microsphere placed on the surface of the object can collect the underlying near-field information, which appears as the evanescent waves and transforms them into propagating waves. Due to the spherical symmetry provided by the microsphere, the super-resolution of the imaging system can be realized in all directions at the same time with one-shot recording. The experiments are carried out for a cosine grating with the line width of 255 nm as the object, which confirms that the lateral resolution can be less than λ/2. Meanwhile, the quantitative phase-contrast image is experimentally obtained. The reconstructed complex field distribution provides the great flexibility with the digital processing for the microscope imaging, such as the ability of refocusing and numerical reconstruction.

Citation Download Citation

Qiaowen Lin, Dayong Wang, Yunxin Wang, Sha Guo, Spozmai Panezai, Liting Ouyang, Lu Rong, and Jie Zhao "Super-resolution quantitative phase-contrast imaging by microsphere-based digital holographic microscopy," Optical Engineering 56(3), 034116 (25 March 2017). https://doi.org/10.1117/1.OE.56.3.034116

Received: 22 December 2016; Accepted: 28 February 2017; Published: 25 March 2017

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