Two-photon microscopy measurement of cerebral metabolic rate of oxygen using periarteriolar oxygen concentration gradients

Sava Sakadžić; Mohammad A. Yaseen; Rajeshwer Jaswal; Emmanuel Roussakis; Anders M. Dale; Richard B. Buxton; Sergei A. Vinogradov; David A. Boas; Anna Devor

doi:10.1117/1.NPh.3.4.045005

17 October 2016 Two-photon microscopy measurement of cerebral metabolic rate of oxygen using periarteriolar oxygen concentration gradients

Sava Sakadžić, Mohammad A. Yaseen, Rajeshwer Jaswal, Emmanuel Roussakis, Anders M. Dale, Richard B. Buxton, Sergei A. Vinogradov, David A. Boas, Anna Devor

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Neurophotonics, Vol. 3, Issue 4, 045005 (October 2016). https://doi.org/10.1117/1.NPh.3.4.045005

Abstract

The cerebral metabolic rate of oxygen (CMRO₂) is an essential parameter for evaluating brain function and pathophysiology. However, the currently available approaches for quantifying CMRO₂ rely on complex multimodal imaging and mathematical modeling. Here, we introduce a method that allows estimation of CMRO₂ based on a single measurement modality—two-photon imaging of the partial pressure of oxygen (PO₂) in cortical tissue. We employed two-photon phosphorescence lifetime microscopy (2PLM) and the oxygen-sensitive nanoprobe PtP-C343 to map the tissue PO₂ distribution around cortical penetrating arterioles. CMRO₂ is subsequently estimated by fitting the changes of tissue PO₂ around arterioles with the Krogh cylinder model of oxygen diffusion. We measured the baseline CMRO₂ in anesthetized rats and modulated tissue PO₂ levels by manipulating the depth of anesthesia. This method provides CMRO₂ measurements localized within ∼200 μm and it may provide oxygen consumption measurements in individual cortical layers or within confined cortical regions, such as in ischemic penumbra and the foci of functional activation.

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Sava Sakadžić, Mohammad A. Yaseen, Rajeshwer Jaswal, Emmanuel Roussakis, Anders M. Dale, Richard B. Buxton, Sergei A. Vinogradov, David A. Boas, and Anna Devor "Two-photon microscopy measurement of cerebral metabolic rate of oxygen using periarteriolar oxygen concentration gradients," Neurophotonics 3(4), 045005 (17 October 2016). https://doi.org/10.1117/1.NPh.3.4.045005

Published: 17 October 2016

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