Photonic neural probe enabled microendoscopes for light-sheet light-field computational fluorescence brain imaging

Peisheng Ding; Hannes Wahn; Fu-Der Chen; Jianfeng Li; Xin Mu; Andrei Stalmashonak; Xianshu Luo; Guo-Qiang Lo; Joyce K. S. Poon; Wesley D. Sacher

doi:10.1117/1.NPh.11.S1.S11503

6 February 2024 Photonic neural probe enabled microendoscopes for light-sheet light-field computational fluorescence brain imaging

Peisheng Ding, Hannes Wahn, Fu-Der Chen, Jianfeng Li, Xin Mu, Andrei Stalmashonak, Xianshu Luo, Guo-Qiang Lo, Joyce K. S. Poon, Wesley D. Sacher

Author Affiliations +

Neurophotonics, Vol. 11, Issue S1, S11503 (February 2024). https://doi.org/10.1117/1.NPh.11.S1.S11503

Abstract

Significance

Light-sheet fluorescence microscopy is widely used for high-speed, high-contrast, volumetric imaging. Application of this technique to in vivo brain imaging in non-transparent organisms has been limited by the geometric constraints of conventional light-sheet microscopes, which require orthogonal fluorescence excitation and collection objectives. We have recently demonstrated implantable photonic neural probes that emit addressable light sheets at depth in brain tissue, miniaturizing the excitation optics. Here, we propose a microendoscope consisting of a light-sheet neural probe packaged together with miniaturized fluorescence collection optics based on an image fiber bundle for lensless, light-field, computational fluorescence imaging.

Aim

Foundry-fabricated, silicon-based, light-sheet neural probes can be packaged together with commercially available image fiber bundles to form microendoscopes for light-sheet light-field fluorescence imaging at depth in brain tissue.

Approach

Prototype microendoscopes were developed using light-sheet neural probes with five addressable sheets and image fiber bundles. Fluorescence imaging with the microendoscopes was tested with fluorescent beads suspended in agarose and fixed mouse brain tissue.

Results

Volumetric light-sheet light-field fluorescence imaging was demonstrated using the microendoscopes. Increased imaging depth and enhanced reconstruction accuracy were observed relative to epi-illumination light-field imaging using only a fiber bundle.

Conclusions

Our work offers a solution toward volumetric fluorescence imaging of brain tissue with a compact size and high contrast. The proof-of-concept demonstrations herein illustrate the operating principles and methods of the imaging approach, providing a foundation for future investigations of photonic neural probe enabled microendoscopes for deep-brain fluorescence imaging in vivo.

CC BY: © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 International License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

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Peisheng Ding, Hannes Wahn, Fu-Der Chen, Jianfeng Li, Xin Mu, Andrei Stalmashonak, Xianshu Luo, Guo-Qiang Lo, Joyce K. S. Poon, and Wesley D. Sacher "Photonic neural probe enabled microendoscopes for light-sheet light-field computational fluorescence brain imaging," Neurophotonics 11(S1), S11503 (6 February 2024). https://doi.org/10.1117/1.NPh.11.S1.S11503

Received: 8 September 2023; Accepted: 20 December 2023; Published: 6 February 2024

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KEYWORDS

Image restoration

Fluorescence imaging

Fluorescence

Brain tissue

Microscopes

Light sources and illumination

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