Multipurpose nonlinear optical imaging system for in vivo and ex vivo multimodal histology

Martin Weinigel; Hans G. Breunig; Aisada Uchugonova; Karsten König

doi:10.1117/1.JMI.2.1.016003

4 March 2015 Multipurpose nonlinear optical imaging system for in vivo and ex vivo multimodal histology

Martin Weinigel, Hans G. Breunig, Aisada Uchugonova, Karsten König

Author Affiliations +

Funded by: German Federal Ministry of Education and Research (BMBF)

Journal of Medical Imaging, Vol. 2, Issue 1, 016003 (March 2015). https://doi.org/10.1117/1.JMI.2.1.016003

Abstract

We report on a flexible multipurpose nonlinear microscopic imaging system based on a femtosecond excitation source and a photonic crystal fiber with multiple miniaturized time-correlated single-photon counting detectors. The system provides the simultaneous acquisition of e.g., two-photon autofluorescence, second-harmonic generation, and coherent anti-Stokes Raman scattering images. Its flexible scan head permits ex vivo biological imaging with subcellular resolution such as rapid biopsy examination during surgery as well as imaging on small as well as large animals. Above all, such an arrangement perfectly matches the needs for the clinical investigation of human skin in vivo where knowledge about the distribution of endogenous fluorophores, second-harmonic generation–active collagen as well as nonfluorescent lipids is of high interest.

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Martin Weinigel, Hans G. Breunig, Aisada Uchugonova, and Karsten König "Multipurpose nonlinear optical imaging system for in vivo and ex vivo multimodal histology," Journal of Medical Imaging 2(1), 016003 (4 March 2015). https://doi.org/10.1117/1.JMI.2.1.016003

Published: 4 March 2015

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