Algorithms and instrumentation for rapid spatial frequency domain fluorescence diffuse optical imaging

Sang Hoon Chong; Vadim A. Markel; Ashwin B. Parthasarathy; Yihong Ong; Kenneth Abramson; Frank A. Moscatelli; Arjun G. Yodh

doi:10.1117/1.JBO.27.11.116002

8 November 2022 Algorithms and instrumentation for rapid spatial frequency domain fluorescence diffuse optical imaging

Sang Hoon Chong, Vadim A. Markel, Ashwin B. Parthasarathy, Yihong Ong, Kenneth Abramson, Frank A. Moscatelli, Arjun G. Yodh

Author Affiliations +

Journal of Biomedical Optics, Vol. 27, Issue 11, 116002 (November 2022). https://doi.org/10.1117/1.JBO.27.11.116002

Abstract

Significance

Rapid estimation of the depth and margins of fluorescence targets buried below the tissue surface could improve upon current image-guided surgery techniques for tumor resection.

Aim

We describe algorithms and instrumentation that permit rapid estimation of the depth and transverse margins of fluorescence target(s) in turbid media; the work aims to introduce, experimentally demonstrate, and characterize the methodology.

Approach

Spatial frequency domain fluorescence diffuse optical tomography (SFD-FDOT) technique is adapted for rapid and computationally inexpensive estimation of fluorophore target depth and lateral margins. The algorithm utilizes the variation of diffuse fluorescence intensity with respect to spatial-modulation-frequency to compute target depth. The lateral margins are determined via analytical inversion of the data using depth information obtained from the first step. We characterize method performance using fluorescent contrast targets embedded in tissue-simulating phantoms.

Results

Single and multiple targets with significant lateral size were imaged at varying depths as deep as 1 cm. Phantom data analysis showed good depth-sensitivity, and the reconstructed transverse margins were mostly within ∼30 % error from true margins.

Conclusions

The study suggests that the rapid SFD-FDOT approach could be useful in resection surgery and, more broadly, as a first step in more rigorous SFD-FDOT reconstructions. The experiments permit evaluation of current limitations.

CC BY: © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 International License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

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Sang Hoon Chong, Vadim A. Markel, Ashwin B. Parthasarathy, Yihong Ong, Kenneth Abramson, Frank A. Moscatelli, and Arjun G. Yodh "Algorithms and instrumentation for rapid spatial frequency domain fluorescence diffuse optical imaging," Journal of Biomedical Optics 27(11), 116002 (8 November 2022). https://doi.org/10.1117/1.JBO.27.11.116002

Received: 13 June 2022; Accepted: 10 October 2022; Published: 8 November 2022

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