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19 December 2018 Supercontinuum-based nondisruptive scattering analyses of mouse fibroblast L929 cells before and after necrosis
Tobias Baselt, Alexander Kabardiadi-Virkovski, Daniel Ruf, Bryan L. Nelsen, Andrés Fabián Lasagni, Peter Hartmann
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Journal of Biomedical Optics, Vol. 23, Issue 12, 121619 (December 2018). https://doi.org/10.1117/1.JBO.23.12.121619
Abstract
The scattering properties of biological tissue are highly dependent on the structure size, refractive index, and wavelength of the incident light. Furthermore, these scattering characteristics are strongly influenced by movements of the scattering objects. A method is developed to determine the angular- and spectral-resolved scattering properties that enabled the characterization of biological nano- and microscaled cell structures. Nanosecond pulses from a spectrally filtered supercontinuum light source are captured and time-resolved to depress background noise and minimize disruptive effects of the biological cells. The scattering characteristics of a monolayer of mouse fibroblast L929 cells are measured at defined wavelengths in a standard cell culture plate. Because of the size and distribution of the scattering structures, a Fourier transform-based Mie scattering scheme is used to analyze the data. The system is tested to detect structural changes of mouse fibroblast L929 cells before and after poisoning with Triton X100. The final result is the development of a contamination-free method to study pathological changes in cell cultures, necrosis, or other cell-damaging effects.
CC BY: © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.
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Tobias Baselt, Alexander Kabardiadi-Virkovski, Daniel Ruf, Bryan L. Nelsen, Andrés Fabián Lasagni, and Peter Hartmann "Supercontinuum-based nondisruptive scattering analyses of mouse fibroblast L929 cells before and after necrosis," Journal of Biomedical Optics 23(12), 121619 (19 December 2018). https://doi.org/10.1117/1.JBO.23.12.121619
Received: 2 July 2018; Accepted: 29 October 2018; Published: 19 December 2018
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KEYWORDS
Scattering
Mie scattering
Light scattering
Biological research
Scatter measurement
Light sources
Sensors
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