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10 October 2017 Second harmonic generation for collagen I characterization in rectal cancer patients with and without preoperative radiotherapy
Stéphanie Blockhuys, Nisha Rani Agarwal, Camilla Hildesjö, Ingvar Jarlsfelt, Pernilla Wittung-Stafshede, Xiao-Feng Sun
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Journal of Biomedical Optics, Vol. 22, Issue 10, 106006 (October 2017). https://doi.org/10.1117/1.JBO.22.10.106006
Abstract
Rectal cancer is treated with preoperative radiotherapy (RT) to downstage the tumor, reduce local recurrence, and improve patient survival. Still, the treatment outcome varies significantly and new biomarkers are desired. Collagen I (Col-I) is a potential biomarker, which can be visualized label-free by second harmonic generation (SHG). Here, we used SHG to identify Col-I changes induced by RT in surgical tissue, with the aim to evaluate the clinical significance of RT-induced Col-I changes. First, we established a procedure for quantitative evaluation of Col-I by SHG in CDX2-stained tissue sections. Next, we evaluated Col-I properties in material from 31 non-RT and 29 RT rectal cancer patients. We discovered that the Col-I intensity and anisotropy were higher in the tumor invasive margin than in the inner tumor and normal mucosa, and RT increased and decreased the intensity in inner tumor and normal mucosa, respectively. Furthermore, higher Col-I intensity in the inner tumor was related to increased distant recurrence in the non-RT group but to longer survival in the RT group. In conclusion, we present a new application of SHG for quantitative analysis of Col-I in surgical material, and the first data suggest Col-I intensity as a putative prognostic biomarker in rectal cancer.
CC BY: © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.
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Stéphanie Blockhuys, Nisha Rani Agarwal, Camilla Hildesjö, Ingvar Jarlsfelt, Pernilla Wittung-Stafshede, and Xiao-Feng Sun "Second harmonic generation for collagen I characterization in rectal cancer patients with and without preoperative radiotherapy," Journal of Biomedical Optics 22(10), 106006 (10 October 2017). https://doi.org/10.1117/1.JBO.22.10.106006
Received: 13 June 2017; Accepted: 12 September 2017; Published: 10 October 2017
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KEYWORDS
Tumors
Cancer
Second-harmonic generation
Tissues
Collagen
Harmonic generation
Optical fibers
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